Words similar to gs-e

Example sentences for: gs-e

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Treatment of luciferase clone 5B3 with 15 μM GS-E for 48 hours consistently induced luciferase levels from near background with only DMSO added, to nearly 150-fold (Table 1) when adjusted for non specific R-Luc induction which was approximately 1.2 fold.
The sensitivity of the gene expression system to varying doses of GS-E was determined by treating duplicate samples of clone MBT-2+RHeoSwitch-2+luc-5B3 with varying concentrations of GS-E for 24 or 48 hours and dual luciferase assays were performed on the cell lysates (Figure 3B).
When the clones had reached at least 50% confluence in the 24 well plate, GS-E was again added to 5 μM to verify the earlier observation of induced EGFP expression.
These cell clones were plated in a six well plate and triplicate wells were treated with DMSO or 15 μM GS-E for 48 hours.
Aliquots of the pooled cells used for cloning were also plated in triplicate, treated with GS-E or DMSO for 24 hrs.