Words similar to goat-anti-rabbit
Example sentences for: goat-anti-rabbit
How can you use “goat-anti-rabbit” in a sentence? Here are some example sentences to help you improve your vocabulary:
The blot was washed once for 5 minutes in PBS-T, incubated with goat-anti-rabbit conjugated HRP (1:10000 dilution) for 1 hour, washed for 5 minutes in PBS-T and developed with Western Lightning Chemiluminescence Reagent Plus (PerkinElmer Life Sciences, Inc.
The membranes were then incubated for 1 hour with goat-anti-rabbit or goat-anti-mouse alkaline-phosphatase-conjugated secondary antibodies (Promega Corp., Madison, WI, USA) diluted 10,000 times with 0.1% gelatin-TNT.
The slides were washed with PBS and secondary antibody (goat-anti-rabbit FITC conjugated) was applied for 20-30 min at room temperature, washed and the signals captured and analyzed using an Olympus BX-50 microscope with Fluoview software.
Blots were developed using a 1:100 dilution of the rabbit anti-hamster IBAT antibodies and an amplified alkaline phosphatase goat-anti-rabbit immunoblot assay kit (Biorad, Hercules, CA) as recommended by the manufacturer.