Words similar to goat
Example sentences for: goat
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Free floating sections were incubated in a combination of rabbit anti β-galactosidase at a dilution of 1/1 k and goat anti ChAT at a dilution of 1/100, overnight at 4°C followed by a combination of donkey anti rabbit tagged with tetramethyl rhodamine isothiocyanate (TRITC) and donkey anti goat tagged with aminomethylcoumarin acetate (AMCA) each at a dilution of 1/50, at room temperature for 60 minutes.
A goat anti-mouse IgG secondary antibody conjugated to Cy3 was used at 1:200 dilution for 1 hour, regardless of which primary antibody was used.
Endogenous peroxidase activity was blocked with 0.1 mol sodium azide containing 1% hydrogen peroxide, and the specimens were incubated with 10% normal goat serum, 2% normal rat serum, and 1% bovine serum albumin for 30 min at room temperature to eliminate non-specific binding.
These studies include entry into J774 macrophages [ 7 8 ] , an electron microscopic examination of goat tissue [ 9 10 ] and an assessment of intracellular fate of M. paratuberculosis within bovine monocytes/macrophages [ 11 12 ] . However, many assumptions regarding M. paratuberculosis interactions with macrophages are based on analogies to M. tuberculosis [ 13 ] or M. avium [ 14 ] . In this communication, we present an analysis of M. paratuberculosis survival within J774 macrophages using transmission electron microscopy to show temporal events early during infection.
Many cloned animals have been created using M II oocytes as the recipient cytoplasm (sheep [ 1, 2], cattle [ 3, 4, 5], mouse [ 6, 7], goat [ 8] and pig [ 9, 10, 11]).
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