Example sentences for: glycosidase

How can you use “glycosidase” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • A doublet of monomers has been previously observed for VR1 expressed in CHO cells [ 23 ] . In that case, treatment of cells with peptide- N -glycosidase F eliminated the larger monomer band, suggesting that it represented a glycosylated form of VR1.

  • Aqueous humor from human, monkey and rabbit was subjected to deglycosylation with a mixture of N-linked and O-linked glycosidases (PNGase F, sialidase, β-galactosidase, glucosaminidase and O-glycosidase) using the Enzymatic CarboRelease Kit according to the manufacturers instructions (QA-Bio).

  • A positive control was not reported in their deglycosylation experiment, so their conclusion that myocilin is not glycosylated may simply be due to inactive glycosidase.

  • Variations in glycosylation patterns have been shown to exist among tissues [ 31 ] and glycosylation levels have been reported to influence hyaluronidase activity of SPAM1 in the mouse and horse [ 10 32 ] . Note that in the mouse, tissue differences in glycosylation patterns have been reported for the testis and epididymis [ 33 ] . It is possible that in the epididymis and vas deferens instead of hyaluronidase activity, a more important role of SPAM1 (which is a glycosidase) may be to modify surface proteins of sperm during their maturation.

  • AH samples in buffer were boiled for 5 min in the presence of denaturant followed by incubation for 3 hr at 37°C with the glycosidase mixture.


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