Words similar to glycosidase
Example sentences for: glycosidase
How can you use “glycosidase” in a sentence? Here are some example sentences to help you improve your vocabulary:
A doublet of monomers has been previously observed for VR1 expressed in CHO cells [ 23 ] . In that case, treatment of cells with peptide- N -glycosidase F eliminated the larger monomer band, suggesting that it represented a glycosylated form of VR1.
Aqueous humor from human, monkey and rabbit was subjected to deglycosylation with a mixture of N-linked and O-linked glycosidases (PNGase F, sialidase, β-galactosidase, glucosaminidase and O-glycosidase) using the Enzymatic CarboRelease Kit according to the manufacturers instructions (QA-Bio).
A positive control was not reported in their deglycosylation experiment, so their conclusion that myocilin is not glycosylated may simply be due to inactive glycosidase.
Variations in glycosylation patterns have been shown to exist among tissues [ 31 ] and glycosylation levels have been reported to influence hyaluronidase activity of SPAM1 in the mouse and horse [ 10 32 ] . Note that in the mouse, tissue differences in glycosylation patterns have been reported for the testis and epididymis [ 33 ] . It is possible that in the epididymis and vas deferens instead of hyaluronidase activity, a more important role of SPAM1 (which is a glycosidase) may be to modify surface proteins of sperm during their maturation.
AH samples in buffer were boiled for 5 min in the presence of denaturant followed by incubation for 3 hr at 37°C with the glycosidase mixture.