Words similar to glycerol
Example sentences for: glycerol
How can you use “glycerol” in a sentence? Here are some example sentences to help you improve your vocabulary:
The lysate was placed on top of buffer GF (7% Ficoll 400, w/v, 20% glycerol, 20 mM HEPES (pH = 7.2), 0.5 mM MgCl 2 ). Nuclei were pelleted by spinning at 7000 rpm in a microfuge.
Control and treated cells were lysed by repeated freeze-thaw cycles with buffer containing 10 mM HEPES (pH 7.5), 1.5 mM Mg(OAc) 2 , 1 mM dithiothreitol (DTT), 0.5% NP40, 5% glycerol, 0.5 mM phenylmethylsulfonyl fluoride, and 10 μg/ml each of the protease inhibitors aprotinin, pepstatin, and leupeptin.
The fractions containing approximately equal band intensity of p66 and p51 were dialyzed against a storage buffer (50 mM Tris HCl pH 7.0, 200 mM NaCl and 50% Glycerol) and this enzyme preparation was used in all experiments.
0.2 fmoles of transposon were incubated with 5.0 μL of EZ::TN™ transposase (Epicentre Technologies) in 25% glycerol at 25°C for 30 min.
Lysates were prepared by glass bead disruption in 50 mM Tris Cl pH 7.5, 1 mM DTT, 40% glycerol.