Words similar to glutathione-agarose
Example sentences for: glutathione-agarose
How can you use “glutathione-agarose” in a sentence? Here are some example sentences to help you improve your vocabulary:
Therefore, we used a GST-pulldown assay to determine if the proteins could interact in vitro . p35-C/EBPβ was expressed as a chimeric GST protein and immobilized on glutathione-agarose beads.
Bacteria were lysed, and soluble proteins were recovered using glutathione-agarose beads (Amersham Pharmacia).
GST-T7-Cdc14 (RDB 872) and GST-Cdc14 (RDB 1183) were expressed in E. coli BL21(DE3) pLysS cells, and purified on glutathione-agarose beads (Sigma) as described previously [ 33 ] . His6-T7-Net1(1-341) (Net1N, RDB1153) [ 11 ] was expressed in E. coli BL21(DE3) pLysS cells, and purified on Ni-NTA agarose beads (Qiagen) as described previously for p28 Kix1 [ 34 ] . Purification of His6-Plx1, His6-Plx1(N172), and His6-HA-Cdc5 from baculovirus-infected insect cells followed an earlier protocol [ 24 ] . The concentrations of purified proteins were in the ranges of: Net1N~0.
Each construct was transformed into E. coli strain BL21 (DE3) (Novagen), and protein was expressed, purified by glutathione-agarose affinity chromatography, cleaved from GST and stored in 20 mM Tris (pH 7.6 at 4°C), 50% glycerol, 1 mM EGTA, 0.1% 2-mercaptoethanol, 4 mM MnCl 2 at -20°C as previously described for PP5 [ 18 ] . Recombinant Ppt1p and Ppt1p (1-504) each contain an additional seven residues, Gly-Ser-Gly-Ser-Glu-Phe-Asp, which are remnants of the thrombin-cleavage site.
Equal protein amounts of each cell lysate (usually 100 ug) were incubated for 3 h at 4°C in the presence of purified GST-c-Jun-(1-79) bound to glutathione-agarose beads (2.
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