Words similar to glucocorticoid
Example sentences for: glucocorticoid
How can you use “glucocorticoid” in a sentence? Here are some example sentences to help you improve your vocabulary:
A gene encoding a plant-optimized GFP variant, mGFP5 [ 24], was placed in frame with a LacI -SV40 nuclear localization signal fusion ( GFP-LacI/NLS ) [ 25] and then subcloned into the vector pTA7002 under the control of the glucocorticoid receptor (GR)-responsive promoter near the left border.
First, MCF7 cells were transfected with the pERV3 vector encoding both the RXR protein and an ecdysone/glucocorticoid receptor fusion protein using GenePorter (Gene Therapy Systems).
C/EBP is previously reported to be an important accessory factor for transactivation by several other transcription factors [ 26 ] . C/EBPβ was shown to transactivate the α1-acid glycoprotein gene promoter even when mutated in the DNA-binding domain, and this activation appeared to depend on protein-protein interaction with glucocorticoid receptor through the leucine zipper region [ 27 ] . Recently, a conserved domain in C/EBPα and C/EBPβ was shown to mediate cAMP responsiveness, strengthening the fact that C/EBP may be characterized as a cAMP-responsive nuclear regulator [ 28 29 ] . On the COX-2 promoter in granulosa cells, cAMP-mediated regulation was shown to be mediated primarily by an E-box that bind USF1 and USF2 isoforms in a manner resembling the RIIβ E-box in Sertoli cells [ 30 ] . Transactivation of the COX-2 gene in skin carcinoma cells is highly dependent on this E-box together with a C/EBP binding site that bind C/EBPβ and C/EBPδ [ 31 ] . These data strengthens a model of formation of a cAMP-responsive unit (CRU) containing USF and C/EBP.
Rab24 contains a unique arginine-rich insert, 126EEDRRRRR 133 [ 25 ] in the domain cognate to loop-8 in H-Ras [ 34 ] . The latter sequence is reminiscent of unusual arginine-rich nuclear localization signals that allow proteins like HIV tet and rev to undergo nuclear translocation by association with importin-β, in the absence of importin-α [ 45 46 ] . Since the loop-8 insert in Rab24 lies adjacent to the conserved N(T)KxD nucleotide binding cassette, we hypothesized that conformational changes caused by the D123I substitution might render the Rab24 mutant capable of disrupting the nuclear import machinery [ 47 ] . To test this possibility, we co-expressed mycRab24wt or mycRab24(D123I) with GR-GFP, a fusion between the glucocorticoid receptor and green fluorescent protein.
A mutant rat glucocorticoid receptor that retained hormone binding, nuclear translocation activity, and specific DNA binding activity, but was incapable of transactivation was fused to a S65T mutant of green fluorescent protein to create pGR(Ala)-GFP.
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