Words similar to glu-

Example sentences for: glu-

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Each construct was transformed into E. coli strain BL21 (DE3) (Novagen), and protein was expressed, purified by glutathione-agarose affinity chromatography, cleaved from GST and stored in 20 mM Tris (pH 7.6 at 4°C), 50% glycerol, 1 mM EGTA, 0.1% 2-mercaptoethanol, 4 mM MnCl 2 at -20°C as previously described for PP5 [ 18 ] . Recombinant Ppt1p and Ppt1p (1-504) each contain an additional seven residues, Gly-Ser-Gly-Ser-Glu-Phe-Asp, which are remnants of the thrombin-cleavage site.
Apart from the curious substitution of 15 of 16 nucleotide-proximal residues in Asw, the most dissimilar region of Asw consists of a five amino-acid insertion between strands β1 and β2 and substitution of Gly-Ala-Pro (Asw) for Asp-Glu-Ser (Hint) at the amino-terminal end of helix α2.
Indeed, halobacteria contain millimolar levels of γ-Glu-Cys, but do not convert it further to GSH [ 2].
1% identity to a reported murine sequence (AF011567) [ 18 ] . Differences in the protein sequences are Gly-247, Gly-284 and Cys-450 in our Hyal-1 protein, compared to Arg-247, Glu-284 and Arg-450 in AF011567.
Recent mutational analysis of recombinant PP5 has identified sequences important in autoinhibition [ 19 ] . Mutation of Glu-76 (underlined in the human PP5 in Fig.