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Example sentences for: giardia
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These restrictions further reduced the number of proteins available for time estimation to the following: AK (36 total, 21 constant-rate), BK-o (25, 16), BK-m (7, 5), BC (20, 16), and Giardia -eukaryotes (17, 11).
Our initial goal was to estimate divergence times for the last common ancestor (LCA), the divergence between archaebacteria and eukaryotes (AK), cyanobacteria and closest eubacterial relatives (origin of cyanobacteria, BC), eubacteria and mitochondrial eukaryotes (origin of mitochondria, BK-m), and Giardia and other eukaryotes (GK) (Fig.
The midasin gene in Giardia intestinalis (accession AF494287) was cloned in silico from sets 1-11 of the unassembled genomic nucleotide sequences kindly made available by the Giardia Genome Project [ 11 ] . Regions of conserved amino acid sequence in the human and yeast genes for midasin were used as input to the Tblastn server at Genbank [ 56 ] to identify clones that encoded homologous regions in Giardia.
The overall alignment of the midasin M-domain from humans, yeast and Giardia with other MIDAS-containing proteins (three Mg-chelatases and the I-domains of three integrins) was created by first aligning the midasins, Mg-chelatases and integrins separately with the program T-Coffee [ 58 ] . The resultant three partial alignments were then combined by the profile alignment procedure of ClustalX, as above.
However, the absence of such a basic cluster in the N-terminal domains of Giardia (residues 1-25) and Schizosaccharomyces pombe (data not shown) renders its significance questionable.
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