Words similar to gfp-mhck-a
Example sentences for: gfp-mhck-a
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To understand whether the differential distribution observed on GFP-MHCK-A, -B and -C cells depended on the existence of myosin II, we expressed these kinases in myosin II null cells and compared the localization patterns.
pTX-MKA1, pMKB-GFP, pTX-MKC1 were transformed into either Ax2 cell line [ 37 ] or HS1 myosin null cells, a strain of D. discoideum with its endogenous mhc A gene deleted [ 38 ] to generate GFP-MHCK-A, -B, and -C cells, respectively.
The cells expressing GFP-MHCK-B appeared to have smooth cell edges because the fluorescence did not label the dynamic pseudopods at the leading edge of the cell, compared with that in GFP-MHCK-A cells.
Interestingly, as the cells progressed from the early to late stages of cytokinesis, GFP-MHCK-B appeared to be excluded from the furrow (Fig 7-B), as compared to the appearance of GFP-MHCK-A or GFP-MHCK-C.
Many cells of each transformation were examined (n > 50) and examples of the distribution of GFP-MHCK-A (Fig.
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