Words similar to gfp-laci
Example sentences for: gfp-laci
How can you use “gfp-laci” in a sentence? Here are some example sentences to help you improve your vocabulary:
To verify if the spots in nuclei correspond to the tagged loci, we examined whether the detected subnuclear localization of the expressed GFP-LacI/NLS fusion protein is sensitive to isopropyl-β-D-thiogalactoside (IPTG).
Under this condition, we found that we could obtain spatial information for subnuclear structures with excess unbound GFP-LacI/NLS fusion proteins.
The nuclear contour as well as the shape of the nucleolus, for which GFP-LacI/NLS has relatively low accessibility and which thus appears as an opaque object within the nucleus, can be captured along with the tagged loci positions by single channel excitation.
This study shows that the observed fluorescence spots in lines EL702C and E indeed correspond to specific binding of the induced GFP-LacI/NLS fusion protein to the tagged loci.
Thus, when stable transgenic Arabidopsis plants are treated with the synthetic glucocorticoid dexamethasone (Dex), the expressed fusion proteins would be able to localize to the integrated loci by association with LacOs . This inducible expression system allows controlled expression of a small amount of GFP-LacI/NLS protein for optimal signal-to-noise and to prevent aggregation of GFP fusion proteins in the nuclei as a result of high protein concentrations.