Words similar to gfp-laci
Example sentences for: gfp-laci
How can you use “gfp-laci” in a sentence? Here are some example sentences to help you improve your vocabulary:
Thus, higher levels of accumulation of GFP-LacI/NLS may be required to detect the loci in our system.
Thus, when stable transgenic Arabidopsis plants are treated with the synthetic glucocorticoid dexamethasone (Dex), the expressed fusion proteins would be able to localize to the integrated loci by association with LacOs . This inducible expression system allows controlled expression of a small amount of GFP-LacI/NLS protein for optimal signal-to-noise and to prevent aggregation of GFP fusion proteins in the nuclei as a result of high protein concentrations.
This latter was transformed with pEL700, which is identical to pEL702 except for the absence of LacOs . As pEL700 does not contain the LacOs , the transgenic line EL700S can be used as a negative control for specific association of GFP-LacI/NLS with the LacOs in our experimental system.
To verify if the spots in nuclei correspond to the tagged loci, we examined whether the detected subnuclear localization of the expressed GFP-LacI/NLS fusion protein is sensitive to isopropyl-β-D-thiogalactoside (IPTG).
This study shows that the observed fluorescence spots in lines EL702C and E indeed correspond to specific binding of the induced GFP-LacI/NLS fusion protein to the tagged loci.