Example sentences for: gfp-laci

How can you use “gfp-laci” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • Thus, higher levels of accumulation of GFP-LacI/NLS may be required to detect the loci in our system.

  • Thus, when stable transgenic Arabidopsis plants are treated with the synthetic glucocorticoid dexamethasone (Dex), the expressed fusion proteins would be able to localize to the integrated loci by association with LacOs . This inducible expression system allows controlled expression of a small amount of GFP-LacI/NLS protein for optimal signal-to-noise and to prevent aggregation of GFP fusion proteins in the nuclei as a result of high protein concentrations.

  • This latter was transformed with pEL700, which is identical to pEL702 except for the absence of LacOs . As pEL700 does not contain the LacOs , the transgenic line EL700S can be used as a negative control for specific association of GFP-LacI/NLS with the LacOs in our experimental system.

  • To verify if the spots in nuclei correspond to the tagged loci, we examined whether the detected subnuclear localization of the expressed GFP-LacI/NLS fusion protein is sensitive to isopropyl-β-D-thiogalactoside (IPTG).

  • This study shows that the observed fluorescence spots in lines EL702C and E indeed correspond to specific binding of the induced GFP-LacI/NLS fusion protein to the tagged loci.


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