Words similar to gfp-laci
Example sentences for: gfp-laci
How can you use “gfp-laci” in a sentence? Here are some example sentences to help you improve your vocabulary:
Western blot analysis with anti-GFP polyclonal antibodies (Clonetech) was used to test inducibility of GFP-LacI/NLS in transgenic plants.
This appears to be a result of aggregation of GFP-LacI/NLS fusion proteins with some insoluble materials in plant cells as we do not encounter this problem with recombinant GFP-LacI/NLS produced in bacteria (data not shown).
Thus, higher levels of accumulation of GFP-LacI/NLS may be required to detect the loci in our system.
Thus, when stable transgenic Arabidopsis plants are treated with the synthetic glucocorticoid dexamethasone (Dex), the expressed fusion proteins would be able to localize to the integrated loci by association with LacOs . This inducible expression system allows controlled expression of a small amount of GFP-LacI/NLS protein for optimal signal-to-noise and to prevent aggregation of GFP fusion proteins in the nuclei as a result of high protein concentrations.
Inhibition of chimeric GFP-LacI/NLS protein binding in situto LacOsby IPTG