Words similar to gfp-laci
Example sentences for: gfp-laci
How can you use “gfp-laci” in a sentence? Here are some example sentences to help you improve your vocabulary:
This appears to be a result of aggregation of GFP-LacI/NLS fusion proteins with some insoluble materials in plant cells as we do not encounter this problem with recombinant GFP-LacI/NLS produced in bacteria (data not shown).
To verify if the spots in nuclei correspond to the tagged loci, we examined whether the detected subnuclear localization of the expressed GFP-LacI/NLS fusion protein is sensitive to isopropyl-β-D-thiogalactoside (IPTG).
This latter was transformed with pEL700, which is identical to pEL702 except for the absence of LacOs . As pEL700 does not contain the LacOs , the transgenic line EL700S can be used as a negative control for specific association of GFP-LacI/NLS with the LacOs in our experimental system.
Although the predicted size of GFP-LacI/NLS fusion protein is 67 kDa, we detected several signals between 70 to 30 kDa in addition to a major species of around 81 kDa on our blots for all lines over time after 24 hours.
This study shows that the observed fluorescence spots in lines EL702C and E indeed correspond to specific binding of the induced GFP-LacI/NLS fusion protein to the tagged loci.
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