Words similar to genistein
Example sentences for: genistein
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To determine whether or not tyrosine kinase activity was required for the action of insulin/IGF-1, the tyrosine kinase inhibitor genistein was used.
, daidzein, genistein and equol) were determined in tissues isolated from the frontal cortex and hippocampus (brain structures critical for VSM) along with the amygdala and cerebellum brain regions, by time-resolved fluoroimmunoassay (TR-FIA) using standard methods based on previously published and validated methods for plasma and urine [ 59 60 ] . The brain tissue method (in brief) follows: The brain samples were lyophilized (pooled by treatment, n = 4 per group) from male rats used as breeders that were on the diet treatments from 50 to 120 days of age.
The final assay was carried out as described elsewhere [ 60 61 ] . Equol was determined in the same way genistein and daidzein, using an antiserum to 4'-O-carboxymethyl-equol-bovine serum albumin and the Europium label was synthesized using the same derivative of equol [ 62 ] . The cross-reactivities of the genistein and daidzein antisera with other isoflavone metabolites have been described previously [ 60 ] . The equol antiserum showed a cross reactivity of 0% for genistein, 12% for dihydrogenistein, 4% for dihydrodaidzein and less than 1% for daidzein and its other metabolites.
Estradiol, diethylstilbestrol (DES), tamoxifen, genistein, coumestrol, thyroid hormone, retinoic acid and trichostatin A (TSA) were purchased from Sigma (St.
3, some oocytes were pretreated with 1 μM genistein for 60 minutes or were injected with RAS antibody (0.