Example sentences for: geneticin

How can you use “geneticin” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • Human Embryonic Kidney (HEK) 293 cells were maintained in D-MEM/F-12 (Dulbecco's Modified Eagle's Medium: Nutrient Mixture F-12 1:1 mixture), supplemented with 10% (v/v) fetal calf serum (FCS), 200 μg/ml G-418 (Geneticin ®) in a humidified incubator with 5% CO 2 and 95% air, at 37°C.

  • Adherent cells were transduced with an amphotropic retrovirus, DFG-hIL-1β-neo, which encodes the mature form of human IL-1β fused to the leader sequence of human parathyroid hormone to enable efficient secretion, and neomycin phosphotransferase [ 21 ] . Retroviral transductants were positively selected in complete DMEM containing Geneticin at 0.5 mg/ml.

  • Stable clones were selected using 400 μg/ml Geneticin ®. Single clone were picked and tested for expression of δ opioid receptor by binding assay.

  • A stable BS-C-1 cell line was made by transfection of GFP-Arp3 (gift from D. Schafer as described [ 34 ] ) using Fugene6 (Roche) and selection with 500 ug/ml Geneticin.


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