Words similar to geneticin
Example sentences for: geneticin
How can you use “geneticin” in a sentence? Here are some example sentences to help you improve your vocabulary:
Ham's F12 medium, Dulbecco's modified Eagle medium (DMEM), fetal bovine serum (FBS), penicillin-streptomycin, type II collagenase, dispase, and Geneticin™ were supplied by Gibco-BRL (Rockville, MD, USA).
Human Embryonic Kidney (HEK) 293 cells were maintained in D-MEM/F-12 (Dulbecco's Modified Eagle's Medium: Nutrient Mixture F-12 1:1 mixture), supplemented with 10% (v/v) fetal calf serum (FCS), 200 μg/ml G-418 (Geneticin ®) in a humidified incubator with 5% CO 2 and 95% air, at 37°C.
All ES cell lines were cultured on mitotically inactivated primary embryonic fibroblasts in ES cell medium supplemented with recombinant leukemia inhibitory factor (LIF) as described elsewhere [ 22 ] . F3 cells were generated by introducing 100 μg of Not1 digested pΔHPRT plasmid into 1.5 × 10 8R1 ES cells by electroporation at 250 volts/500μf/resistance 8 using a BTX ECM600 electroporation system [ 7 ] . Cells containing neo were selected by supplementing the ES cell medium with 250 μg/ml Geneticin (G418, Gibco-BRL) and negative selection against Hsv-tk gene expression was achieved by including 2 μM gancyclovir (Roche).
Transfection of UMUC3 cells with pUHD172-1neo was followed 48 hr later by selection using geneticin.
G418-resistant cell lines were selected in DMEM + 10% fetal bovine serum (Clontech, Tet-free) + 200 μg/ml Geneticin (Invitrogen).