Words similar to geneamp
Example sentences for: geneamp
How can you use “geneamp” in a sentence? Here are some example sentences to help you improve your vocabulary:
PCR was performed on a Perkin-Elmer 9600 GeneAmp PCR system, as specified in the HybriZAP™ Two-Hybrid cDNA Gigapack Cloning Kit instruction manual (Stratagene).
This was then amplified by 35 to 50 cycles of the polymerase chain reaction (PCR, GeneAmp RNA PCR kit, N808-0143, Perkin Elmer, Foster City, CA), with primers (Table 1) designed for osteocalcin, type I collagen α1, type II collagen, BMP-2, BMP-4, type IA BMP receptor, 18S ribosomal RNA, or G3PDH (Cat.
PCR amplifications of cDNA inserts were carried out at annealing temperatures of 50°C (614 and 606 libraries) or 60°C (707 library) in a 25 μl volume in a GeneAmp PCR system 9700 (Applied Biosystems, Foster City, CA) thermocycler for 35 cycles with a 2 min extension time.
All test PCRs were carried out using 30 ng of template DNA with 1.5 μl GeneAmp 10 × PCR buffer II (Roche Molecular Systems, Inc.), either 1.5 mM or 3.0 mM Mg 2+, 200 μM dNTPs, 0.2 μM each forward and reverse primer, and 0.375 units of AmpliTaq Gold Polymerase (Roche Molecular Systems, Inc.) in a 15 μl final volume.
The amplification was carried out and analysed in the GeneAmp 5700 Sequence Detection System (PE Biosystems).
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