Words similar to geneamp
Example sentences for: geneamp
How can you use “geneamp” in a sentence? Here are some example sentences to help you improve your vocabulary:
Template DNA was amplified by PCR in the Perkin Elmer GeneAmp PCR system 9700.
All test PCRs were carried out using 30 ng of template DNA with 1.5 μl GeneAmp 10 × PCR buffer II (Roche Molecular Systems, Inc.), either 1.5 mM or 3.0 mM Mg 2+, 200 μM dNTPs, 0.2 μM each forward and reverse primer, and 0.375 units of AmpliTaq Gold Polymerase (Roche Molecular Systems, Inc.) in a 15 μl final volume.
Total RNA was purified from dissected nephron segments using Tri Reagent (Molecular Research Center) and reverse-transcribed to single-stranded cDNA using Moloney murine leukemia virus reverse transcriptase and 2.5 μM of random hexamers according to the manufacturer's protocol (GeneAmp RNA PCR kit, Perkin Elmer Cetus, Norwalk, CT).
Electroporation of DNA into Y. pestis was done as described previously [ 32 ] . Gene amplification was performed with DeepVent (New England Biolabs, Beverly, MA) or Taq (Eppendorf Scientific, Westbury, NY) DNA polymerase in a Perkin-Elmer GeneAmp Model 2400 thermocycler (Applied Biosystems, Foster City, CA).
The M6P/IGF2R transcripts were determined by RT-PCR using the GeneAmp EZ rTt RNA PCR kit (Roche).