Words similar to geneamp

Example sentences for: geneamp

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PCR amplifications of cDNA inserts were carried out at annealing temperatures of 50°C (614 and 606 libraries) or 60°C (707 library) in a 25 μl volume in a GeneAmp PCR system 9700 (Applied Biosystems, Foster City, CA) thermocycler for 35 cycles with a 2 min extension time.
All test PCRs were carried out using 30 ng of template DNA with 1.5 μl GeneAmp 10 × PCR buffer II (Roche Molecular Systems, Inc.), either 1.5 mM or 3.0 mM Mg 2+, 200 μM dNTPs, 0.2 μM each forward and reverse primer, and 0.375 units of AmpliTaq Gold Polymerase (Roche Molecular Systems, Inc.) in a 15 μl final volume.
PCR was performed on a Perkin-Elmer 9600 GeneAmp PCR system, as specified in the HybriZAP™ Two-Hybrid cDNA Gigapack Cloning Kit instruction manual (Stratagene).
Electroporation of DNA into Y. pestis was done as described previously [ 32 ] . Gene amplification was performed with DeepVent (New England Biolabs, Beverly, MA) or Taq (Eppendorf Scientific, Westbury, NY) DNA polymerase in a Perkin-Elmer GeneAmp Model 2400 thermocycler (Applied Biosystems, Foster City, CA).
XL-PCR was performed using GeneAmp XL PCR kit (PE Applied Biosystems, Foster City, CA).