Words similar to gelatin
Example sentences for: gelatin
How can you use “gelatin” in a sentence? Here are some example sentences to help you improve your vocabulary:
Precast zymography gels containing 10% Tris glycine with 0.1% gelatin as substrate were purchased from Novex (San Diego, CA, USA), and protein quantification kits were purchased from Bio-Rad Laboratories (Hercules, CA, USA).
Polymerase chain reaction (PCR) was carried out in a total volume of 11 μl containing 0.5 pM of each primer, 0.2 mM dNTP, 2 mM MgCl 2 , 5% dimethysulphoxide (DMSO), 0.275 U Taq polymerase, 50 mM KCl, 10 mM Tris-HCl pH 8.3, and 0.001% gelatin.
Similarly to work previously reported [ 20], constituted extracts, in 1-10 ml 0.2% gelatin or 0.2% HSA, were applied to calibrated 2.5 × 100 cm columns containing G-75 superfine Sephadex (Pharmacia Biotech, Piscataway, New Jersey, USA) suspended in 0.1% human serum albumin (1 g HSA, 0.1 mol NH 4 HCO 3 and 0.1 g merthiolate/l at pH = 7.5) at 4°C.
Mononucleated cells were resuspended in plating medium containing 78% Dulbecco's modified Eagle's medium (DMEM) with low glucose (Life Technologies, Rockville, MD), 10 % horse serum (HS, Sigma, St. Louis, MO), 10 % fetal bovine serum (FBS, Sigma, St. Louis, MO), 2% chicken embryo extract (CEE, Life Technologies, Rockville, MD) and plated on plastic culture dishes coated with gelatin at a density of ∼ 1 × 10 4cells per dish.
PCR amplifications were carried out in a 4 μl volume containing 10 mM Tris-HCl (pH 8.3), 50 mM KCl, 1.5 mM MgCl 2 , 0.001% gelatin, 100 μM each dNTP, 0.075 μM of fluorescent-labelled forward and unlabeled reverse primer, and a 0.12 units of Taq polymerase.
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