Words similar to gelatin
Example sentences for: gelatin
How can you use “gelatin” in a sentence? Here are some example sentences to help you improve your vocabulary:
CAP, cellulose acetate phthalate; STD, sexually transmitted disease; ELISA, enzyme-linked immunosorbent assay; mAbs, monoclonal antibodies; FBS, fetal bovine serum; PEG 6000, polyethylene glycol 6000; HIV-1, human immunodeficiency virus type 1; BSA, bovine serum albumin; PBS, phosphate buffered saline; Ch-PBS, chicken serum (10%) in PBS; PBS-BG, 1% BSA/1% gelatin in PBS; HRP, horseradish peroxidase; sCD4, soluble CD4; TS, 0.14 M NaCl, 0.01 M Tris, pH 7.0; CTAP, cellotetraose acetate phthalate; pdb, Protein Data Bank; ED 50 , effective dose for 50 % inhibition.
1% gelatin, 1% normal goat serum, 0.3% Triton-X-100 in TBS), placed in primary antibody (1:10 diluted in blocking buffer for all antibodies used) for 2 hours at room temperature, rinsed 3 times for 5 min each in TBS, placed in gold-conjugated secondary antibodies (10 nm gold anti-mouse and 5 nm gold anti-rabbit) for 1 hour, rinsed 3 times in TBS, fixed in 2% glutaraldehyde, rinsed 3 times in TBS, rinsed in water, stained with Reynold=s lead citrate followed by aqueous 2% uranyl acetate, dried on filter paper and viewed using a Philips CM10 transmission electron microscope.
Subsequently the wells were washed and postcoated with BSA and gelatin as described above, except that these proteins were dissolved in 0.14 M NaCl, 0.01 M Tris, pH 7.0 (TS).
Recombinant gp120 HIV-1 IIIB in the presence of a 10-fold excess of BSA and gelatin, respectively, was quantitated by adding the gp120/BSA/gelatin mixtures (before and after binding to micronized CAP or Aquateric; Fig.
Briefly, 10 μl of each sample were electrophoresed at 4°C and under non-denaturing conditions using a 10% polyacrylamide gel containing 0.05% gelatin.
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