Words similar to gel-purified
Example sentences for: gel-purified
How can you use “gel-purified” in a sentence? Here are some example sentences to help you improve your vocabulary:
The products of the second PCR amplification were gel-purified and subcloned into pGEM-T (Promega).
The cDNA probe was prepared by restriction digestion of pET-GST-PPT1 with Bsm I and Nco I. The 879 bp DNA fragment generated, corresponding to 544-1422 of PPT1 coding sequence, was gel-purified and labeled with 0.67 μM [α- 32P] CTP (3000 Ci/mmol) using the DECAprimeII random priming kit (Ambion) according to the user manual.
The gel-purified ~400-bp fragment was inserted into the Cla I and Hin cII sites of p3002PCR (the plasmid containing core ars3002 generated by PCR; [ 10]).
The PCR products were gel-purified using low melting temperature agarose and subjected to restriction enzyme digestion with Nde I and Xho I. The same two restriction enzymes were used to digest plasmid pET-24b+ DNA.
All of the PCR products were gel-purified and sequenced to confirm that they contain the expected Fz sequences (data not shown).