Words similar to gdn-
Example sentences for: gdn-
How can you use “gdn-” in a sentence? Here are some example sentences to help you improve your vocabulary:
Fibrosarcoma PAI-1, wtPAI-1 or its mutants were denatured with 6 M guanidine-hydrochloride (Gdn-HCl) (45 min at 37°C), followed by dialysis (4-16 hours at 4°C) against 50 mM sodium acetate, pH 5.6, containing 0.5 M NaCl and 0.01% Tween-20.
Single site mutations within the segment Asp355-Arg356-Pro357 of PAI-1 yield Gdn-HCl activatable inhibitors that can still form SDS stable complexes with plasminogen activators, with second order inhibition constants that are similar to the native inhibitor.
This process which PAI-1 regained its activity is called activation by Gdn-HCl.
4A, the specificity of wtPAI-1, Glu351Ala and Glu351Arg after denatured by 6 M Gdn-HCl and dialyzed against 50 mM sodium acetate, pH 5.6, was 84 ± 15, 112 ± 18 and 68 ± 9 U/ug, respectively.
Gdn-HCl, guanidine-hydrochloride; HRP, horseradish peroxidase; ECL, enhanced chemiluminescence; PBS, phosphate-buffered saline; BSA, bovine serum albumin.