Example sentences for: gct

How can you use “gct” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • For EBV DNA detection two primer pairs EBER 3 (5' gca acg gct gct ctg ttt ga), EBER 5 (5' gtg gtc cgc atg ttt tga tc) and TC60 (5' cca gag gta agt gga ctt), TC61 (5' gac cgg tgc ctt ctt agg) were used.

  • A cDNA that encodes a hemagglutinin-tagged NPM3 protein was produced by PCR under the following conditions: 100 μl reactions with 1X Pfu buffer, 0.2 mM deoxyribonucleotides, 1 μM HA-F primer (5'-AAA GAA TTC AGC ATG TAC CCA TAC GAC GTC CCA GAC TAC GCC GCC GCC GGT ACT GCA GCT GCC-3'), 1 μM NPM3 -R2 primer (5'-AAA GAA TTC CTA GGG CCT GCC CCC CTG CTT TTT GGC AGG AAG GAT GGG-3'), 1 ng of human NPM3 cDNA insert, and 2.5 Units of recombinant Pfu DNA Polymerase.

  • Nine cell lines derived from GCT have been previously described [ 8 ] . DNA and RNA isolated from four normal testes were used as controls.

  • Therefore, these data indicate that other epigenetic and/or genetic changes may be involved in regulating the expression of MGMT in GCT.

  • We have previously shown that subsets of resistant tumors exhibit TP53 gene mutations and chromosomal amplifications [ 6 ] . However, the role of MGMT in GCT sensitivity or resistance to chemotherapy is not known.


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