Words similar to gct

Example sentences for: gct

How can you use “gct” in a sentence? Here are some example sentences to help you improve your vocabulary:

A cDNA that encodes a hemagglutinin-tagged NPM3 protein was produced by PCR under the following conditions: 100 μl reactions with 1X Pfu buffer, 0.2 mM deoxyribonucleotides, 1 μM HA-F primer (5'-AAA GAA TTC AGC ATG TAC CCA TAC GAC GTC CCA GAC TAC GCC GCC GCC GGT ACT GCA GCT GCC-3'), 1 μM NPM3 -R2 primer (5'-AAA GAA TTC CTA GGG CCT GCC CCC CTG CTT TTT GGC AGG AAG GAT GGG-3'), 1 ng of human NPM3 cDNA insert, and 2.5 Units of recombinant Pfu DNA Polymerase.
The introns were PCR-amplified with KM37 (GAT AAT ACG ACT CAC TAT AAT GGC ATT ACC GCC TTG T) and GM24 (GCT CTA GAC TTA GCT ACA ATA TGA AC) in 25 μl reactions under the conditions stated above and cycled 20 times.
Since a number of gene promoters were hypermethylated and showed down-regulated mRNA in GCT, we wanted to test whether hypomethylation reactivates the gene expression in these tumors.
In the selection in which both the promoter and the polymerase pool was used, T7 RNA polymerase was similarly randomized at amino acid positions 748, 756, and 758 using the oligonucleotide gcT7lib2.
6 and gcT7lib1) and (gcT7a.