Words similar to gal-
Example sentences for: gal-
How can you use “gal-” in a sentence? Here are some example sentences to help you improve your vocabulary:
The following plasmids (pSG5-ERα, pSG5-ERβ (amino acids 1-530) [ 31 ] , pGEX-N-CoR and pGEX-SMRT [ 39 ] , VP16-TRβ and Gal-N-CoR [ 16 ] , GST-N-CoR fusions [ 17 ] , ERE-LUC, GK1/Gal4 responsive reporter and Gal-ERα LBD [ 53 ] , pM-D2, pM-D47, pM-F6 [ 46 ] ) have been previously described.
VP16-ERβ LBD and Gal-ERβ LBD contain human ERβ sequences and were gifts from Dr. Dale Leitmann (University of California, San Francisco).
VP16-ERβ mutations and Gal-N-CoR mutations were prepared using standard PCR-based site directed mutagenesis (Quickchange Kit, Stratagene, La Jolla, CA) and confirmed by sequencing.
Gal-GRIP1 NR box (1,2,3) fusion (amino acids 610-770) was prepared by PCR amplification of the appropriate region of GRIP1 (primers obtained from Biomolecular Resource Center, UCSF) containing EcoRI and SalI sites, the PCR fragment was digested with these enzymes and subcloned into the pM GAL4 expression vector (Clontech Laboratories, Inc.