Words similar to gal
Example sentences for: gal
How can you use “gal” in a sentence? Here are some example sentences to help you improve your vocabulary:
To begin to test this hypothesis, we used a GAL4 dependent reporter construct, in which a CAT reporter gene is driven by five copies of a GAL4 binding site upstream of the adenovirus E1B minimal promoter (pG5CAT).
Since genes encoding highly conserved activites often function across species boundaries, we attempted to evaluate mouse Cul1 (mCul1) function in Drosophila . To this end, we used a GAL4 transgene to drive overexpression of mCul1 in the wing disc.
We also tested if an nrg1Δ affects glucose repression of the GAL genes as described in Materials and Methods.
After rinsing with PBS, cells were incubated in the dark (20 h at 37°C) in a buffer containing X-gal substrate (1 mg/ml), potassium ferricyanide (100 mM), and potassium ferrocyanide (100 mM).
When CMV-LAP (which encodes p35-C/EBPβ) is transfected with GAL4-SRF and the reporter construct, we see a 7-fold increase in CAT activity that is potentiated to 75-fold when activated Ras is cotransfected.