Words similar to foa
Example sentences for: foa
How can you use “foa” in a sentence? Here are some example sentences to help you improve your vocabulary:
The resulting colonies were then grown in YPAD media (1% yeast extract, 2% peptone, 0.04% adenine, and 2% glucose) and plated on 5-FOA plates to select clones emerging by a second recombination event that eliminated the URA3 gene together with the additional copy of PPT1 . To confirm gene replacement, chromosomal DNA from these clones was used for PCR amplification of the region encoding HA-Ppt1p.
To introduce tab mutations into the wild type background, cdc15Δ tab strains were crossed to CDC15 TAB, and tab spores were verified by crossing to cdc15Δ [pMET3-CDC15, URA3], and demonstrating that half of the resulting cdc15Δ segregants were 5-FOA resistant.
Each transformant was assayed for the occurrence of correct integration and its ability to survive on 5-FOA.
The resulting YAC does not express URA3 and, therefore, the clone can be selected by medium containg 5-FOA.
If the TAR vector recombines with a genomic DNA fragment, the URA3 gene is lost and the resulting YAC clone becomes resistant to 5-FOA.