Example sentences for: foa

How can you use “foa” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • To introduce tab mutations into the wild type background, cdc15Δ tab strains were crossed to CDC15 TAB, and tab spores were verified by crossing to cdc15Δ [pMET3-CDC15, URA3], and demonstrating that half of the resulting cdc15Δ segregants were 5-FOA resistant.

  • If the TAR vector recombines with a genomic DNA fragment, the URA3 gene is lost and the resulting YAC clone becomes resistant to 5-FOA.

  • The diploids were sporulated, and if ~50% of the MEN-deleted spores were resistant to 5-FOA, then net1-1 could bypass the MEN null mutation.

  • We mutated an out-of-frame ATG codon identified in a B1 template sequence during PCR amplification of the hook to avoid translational incompetence and resistance to 5-FOA.

  • The targeting hooks were placed in the promoter region of URA3 as shown in Figure 2. Genomic DNA and the linearized TAR cloning vector were combined with yeast spheroplasts and the His +Ura -transformants were selected on medium containing 5-FOA to identify clones with genomic DNA inserts.


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