Words similar to foa

Example sentences for: foa

How can you use “foa” in a sentence? Here are some example sentences to help you improve your vocabulary:

The resulting YAC does not express URA3 and, therefore, the clone can be selected by medium containg 5-FOA.
Optimum conditions for TAR cloning have been recently described [ 30 ] . Transformants were selected on synthetic histidine-minus plates containing 5-fluoroorotic acid (5-FOA) to select clones that no longer express URA3 [ 31 ] . Because of its toxic effect on yeast spheroplasts, 5-FOA was not included in the top agar.
His +Ura -transformants were selected on medium containing 5-FOA to identify recombinant clones.
Expression of URA3 (determined by the sensitivity of His +transformants to 5-FOA) was abolished with 150-bp and 190-bp Alu inserts.
The resulting colonies were then grown in YPAD media (1% yeast extract, 2% peptone, 0.04% adenine, and 2% glucose) and plated on 5-FOA plates to select clones emerging by a second recombination event that eliminated the URA3 gene together with the additional copy of PPT1 . To confirm gene replacement, chromosomal DNA from these clones was used for PCR amplification of the region encoding HA-Ppt1p.