Words similar to foa
Example sentences for: foa
How can you use “foa” in a sentence? Here are some example sentences to help you improve your vocabulary:
pURA4- rec12-Y98F was linearized by digestion with Nco I. Transformation, forward selection for Uracil prototrophy, and reverse selection for FOA resistance were as described [ 61 63 ] . Candidates were screened as described above.
Circularization of the vector does not prevent URA3 expression and, therefore, the clones containing the vector without the DNA insert can not grow on media containing 5-FOA.
The targeting hooks were placed in the promoter region of URA3 as shown in Figure 2. Genomic DNA and the linearized TAR cloning vector were combined with yeast spheroplasts and the His +Ura -transformants were selected on medium containing 5-FOA to identify clones with genomic DNA inserts.
The resulting YAC does not express URA3 and, therefore, the clone can be selected by medium containg 5-FOA.
Expression of URA3 (determined by the sensitivity of His +transformants to 5-FOA) was abolished with 150-bp and 190-bp Alu inserts.