Words similar to foa

Example sentences for: foa

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Optimum conditions for TAR cloning have been recently described [ 30 ] . Transformants were selected on synthetic histidine-minus plates containing 5-fluoroorotic acid (5-FOA) to select clones that no longer express URA3 [ 31 ] . Because of its toxic effect on yeast spheroplasts, 5-FOA was not included in the top agar.
Both selected targeting sequences do not contain an ATG initiation codon that is out-of-frame with the vector URA3 , which avoids the translational incompetence of URA3 mRNA and resistance to 5-FOA (see Methods).
We mutated an out-of-frame ATG codon identified in a B1 template sequence during PCR amplification of the hook to avoid translational incompetence and resistance to 5-FOA.
The resulting YAC does not express URA3 and, therefore, the clone can be selected by medium containg 5-FOA.
His +Ura -transformants were selected on medium containing 5-FOA to identify recombinant clones.