Words similar to first-strand

Example sentences for: first-strand

How can you use “first-strand” in a sentence? Here are some example sentences to help you improve your vocabulary:

First-strand cDNA synthesis was primed with 4 μg of random nonamers (New England Biolabs) or 6 μg random hexamers (Life Technologies) by heating at 70°C for 10 minutes, snap-cooling in dry ice/ethanol for 30 sec, and incubating at room temperature for an additional 5-10 min.
Second-strand synthesis was primed by the partially digested RNA that resulted from RNase H treatment of the first-strand synthesis reaction.
This second round of cDNA synthesis was similar to the first round except that random hexamers were used in priming of first-strand synthesis, with T7-(dT) 24 oligomer priming the second-strand.
For the hybridization experiments, 12 μg total RNA was used for first-strand cDNA synthesis as follows: RNA was mixed with a mixture of random hexamer (pdN 6 ) oligonucelotides and oligo-(dT 20 ) at final concentration 125 μg/μl for each oligonucleotide.
It is important to note that our protocol for generating first-strand cDNA target involves the use of random primers.