Words similar to first-strand
Example sentences for: first-strand
How can you use “first-strand” in a sentence? Here are some example sentences to help you improve your vocabulary:
First-strand cDNA was prepared, labeled and processed as described in the Motorola CodeLink system protocols.
We indirectly labeled the hybridization probes by incorporation of amino-allyl modified nucleotides in a first-strand cDNA RT reaction.
Second-strand synthesis was primed by the partially digested RNA that resulted from RNase H treatment of the first-strand synthesis reaction.
First-strand cDNA synthesis was primed from the 3' end of the poly(A) RNA using a poly(T) primer that also contained an Xho I restriction site and a GAGA sequence (5'-GAGAGAGAGAGAGAGAGAGAACTAGTCTCGAGTTTTTTITTTTTTTTTTT-3').
Six microliters first-strand premix were added and incubated at 42°C for 2 h (6 μl mix was made up with 2 μl 5× first-strand buffer, 1 μl 0.1 M DTT, 1 μl dNTPs (10 mM each), 0.3 μl T4gp32 (USB, 13.