Words similar to first-strand

Example sentences for: first-strand

How can you use “first-strand” in a sentence? Here are some example sentences to help you improve your vocabulary:

First-strand cDNA was prepared, labeled and processed as described in the Motorola CodeLink system protocols.
The T7-(dT) 24 oligomer was used for priming first-strand cDNA synthesis.
As first-strand cDNA synthesis is identical for both samples, incorporation of nucleotides with an aminoallyl linker followed by the covalent coupling reaction to Cy dyes reduces bias for incorporation of one dye over the other by the reverse transcriptase.
First-strand cDNA was purified from unincorporated amino allyl-dUTPs on QIAquick PCR purification columns (Qiagen, Valencia, CA) according to manufacturer's instructions, except that QIAquick wash buffer was replaced with 5 mM K +phosphate buffer (pH 8.5) containing 80% ethanol, and cDNA was eluted with 4 mM K +phosphate buffer (pH 8.5).
mRNA (150 ng) was reverse transcribed for each muscle sample with the First-strand cDNA Synthesis Kit (Amersham Pharmacia, Piscataway, New Jersey), and the cDNA product was then used in the PCR.