Words similar to first-strand
Example sentences for: first-strand
How can you use “first-strand” in a sentence? Here are some example sentences to help you improve your vocabulary:
As first-strand cDNA synthesis is identical for both samples, incorporation of nucleotides with an aminoallyl linker followed by the covalent coupling reaction to Cy dyes reduces bias for incorporation of one dye over the other by the reverse transcriptase.
First-strand cDNA synthesis was primed with 4 μg of random nonamers (New England Biolabs) or 6 μg random hexamers (Life Technologies) by heating at 70°C for 10 minutes, snap-cooling in dry ice/ethanol for 30 sec, and incubating at room temperature for an additional 5-10 min.
5-methyl dCTP was used during first-strand cDNA synthesis to protect internal Xho I sites.
After synthesis of the first-strand cDNA, PCR was performed by initial denaturing at 94°C for 2 min followed by 28 cycles of denaturation at 94°C for 1 min, annealing at 60°C for 1 min, and extension at 72°C for 1.5 min.
DNA and RNA preparation, first-strand cDNA synthesis
Loading...