Words similar to first-strand

Example sentences for: first-strand

How can you use “first-strand” in a sentence? Here are some example sentences to help you improve your vocabulary:

Six microliters of 5x first-strand buffer (RGI) (1 μl 100 mM DTT, 1.5 μl dNTPs (20 mM), 1.5 μl reverse transcriptase (Superscript II, Gibco BRL), 10 μl [ 33P]dCTP (ICN)) were then added to the primed RNA.
Reverse transcription was performed in the presence of 500 μM each of dATP, dCTP, and dGTP, 200 μM 5-aminoallyl-dUTP (Sigma), 300 μM dTTP, 1 × first-strand buffer, 10 mM dithiothreitol, and 400 U Superscript II (Life Technologies) in 40-μl reactions at 42°C for 3 h to overnight.
Total RNA was obtained from mouse embryonic tissue or adult mouse organs and reverse transcription carried out as described [ 34 ] . For each tissue analysis, PCR was carried out in a 25 μl reaction volume using 1 μl of the first-strand cDNA reaction, according to the Superscript Preamplification System for First Strand cDNA Synthesis (Invitrogen).
mRNA (150 ng) was reverse transcribed for each muscle sample with the First-strand cDNA Synthesis Kit (Amersham Pharmacia, Piscataway, New Jersey), and the cDNA product was then used in the PCR.
The mix was incubated at 65°C for 10 min before 5 μl first-strand premix was added followed by incubation at 42°C for 2 h.