Words similar to first-strand

Example sentences for: first-strand

How can you use “first-strand” in a sentence? Here are some example sentences to help you improve your vocabulary:

A reaction mixture consisting of mRNA, first-strand buffer (Life Technologies), DTT (0.
Total RNA samples (1 μg) were reverse transcribed to yield first-strand cDNA using the Applied Biosystems Reverse Transcription Reagents protocol (Applied Biosystems, Foster City, CA, USA).
After synthesis of the first-strand cDNA, PCR was performed by initial denaturing at 94°C for 2 min followed by 28 cycles of denaturation at 94°C for 1 min, annealing at 60°C for 1 min, and extension at 72°C for 1.5 min.
5-methyl dCTP was used during first-strand cDNA synthesis to protect internal Xho I sites.
First-strand cDNA synthesis was primed from the 3' end of the poly(A) RNA using a poly(T) primer that also contained an Xho I restriction site and a GAGA sequence (5'-GAGAGAGAGAGAGAGAGAGAACTAGTCTCGAGTTTTTTITTTTTTTTTTT-3').