Words similar to first-strand

Example sentences for: first-strand

How can you use “first-strand” in a sentence? Here are some example sentences to help you improve your vocabulary:

Second-strand synthesis was primed by the partially digested RNA that resulted from RNase H treatment of the first-strand synthesis reaction.
With the mixture remaining on ice, 4 μl of 5X first-strand buffer, 2 μl 0.1 M DTT, 1 μl of 10 mM dNTP mix and 1 μl Superscript™ II RNaseH -reverse transcriptase (200 U/μl) was added to make a final volume of 20 μl, and the mixture incubated for one hour in a 42°C water bath.
mRNA (150 ng) was reverse transcribed for each muscle sample with the First-strand cDNA Synthesis Kit (Amersham Pharmacia, Piscataway, New Jersey), and the cDNA product was then used in the PCR.
It is important to note that our protocol for generating first-strand cDNA target involves the use of random primers.
As first-strand cDNA synthesis is identical for both samples, incorporation of nucleotides with an aminoallyl linker followed by the covalent coupling reaction to Cy dyes reduces bias for incorporation of one dye over the other by the reverse transcriptase.