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Example sentences for: fbs

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AMPA, α-amino-3-hydroxy-5-methyl-4-isoxazole propionic acid; DMEM, Dulbecco's modified Eagle medium; FBS, fetal bovine serum certified; FCC, ferricytochrome c type III; HBSS, Hank's balanced salt solution; LPS, lipopolysaccharide; MMP-9, matrix metalloproteinase-9; O 2 -, superoxide; P, penicillin; PBS, phosphate buffered saline; PMA, phorbol 12-myristate 13-acetate ; RT-PCR, reverse transcriptase polymerase chain reaction; S, streptomycin; SOD, superoxide dismutase; TNF-α, tumor necrosis factor.
To test density effects, cells were plated in duplicate at initial numbers of 1, 2, 5, 10, 20 and 40 × 10 5per 100 mm cell culture dish with DMEM containing 5% FBS and 5% calf serum.
Infections were typically at an MOI of 0.01 PFU/cell in Medium 199 plus 2% FBS.
After microinjection the cells were incubated in medium containing 10% FBS for 6 hours at 37°C.
8-5 × 10 6cells/60 mm × 15 mm polystyrene cell culture dish) were treated with (1) 1 mM domoic acid, (2) LPS-free water used as a vehicle for domoic acid or (3) 10 ng/ml LPS in a final volume of 5 ml of DMEM supplemented with 10% FBS + 120 U/ml P + 12 μg/ml S and incubated in a humidified 5% CO 2 incubator at 36°C for 1 to 6 h.