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Example sentences for: fb

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Using these membranes, filtration binding assays were performed according to previously described protocols [ 28 ] . To reduce nonspecific binding, 96-well filtration plates (fiberglass FB filters; Millipore, Bedford, USA) were pre-coated as suggested by the manufacturer with 0.5% polyethyleneimine + 0.1% BSA and then washed four times with 0.1% BSA.
To sequence the junction fragments from both strands, we performed PCR and sequencing with primers 774F and reverse primer E43+355 (5'-CAA AGT CTC TGT CTG GAA GAG AAA TGA TTT TGA-3') for FB774, and with forward primer E44 -343 (5'-CCT TAG CTT CCC AAA GTG CTA GGA TTA-3') and 890R for FB890.
A reacting protein band (at the molecular weight of 75 kD, as indicated by the company, http://www.upstate.com) was seen in wild-type cell lines expressing a normal sized MeCP2 protein, and in cell lines with missense mutations (FB R106W, FB R306C and LCL T158M).
No FB elements were identified using RepeatMasker; a more targeted search identified 12 kb (0.
1%) of FB element sequence.