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Example sentences for: fam-

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A specific exon 20 mutation (T790M) was also detected by length analysis of fluorescently labeled (FAM) PCR products on a capillary electrophoresis device (ABI 3100 Avant, Applied Biosystems, Foster City, California, United States), based on a new NlaIII restriction site created by the T790M mutation (2369 C→T), using the following primers: EGFR Ex20F, 5′-FAM- CTCCCTCCAGGAAGCCTACGTGAT-3′ and EGFR Ex20R 5′- TTTGCGATCTGCACACACCA-3′.
P1 is an "Amplifluor™"-primer, with a 5' hairpin and loop structure with a fluorophore and quencher at the base of the hairpin arms [ 13 12 16 ] . The two allele-specific OCPs have different backbone sequences complementary either to a FAM- or TET- labeled P1 allowing discrimination of the polymorphic base.
In 12 cases, exon 19 deletions were also studied by length analysis of fluorescently labeled PCR products on a capillary electrophoresis device, using the following primers: EGFR -Ex19-FWD1, 5′- GCACCATCTCACAATTGCCAGTTA-3′, and EGFR -Ex19-REV1, 5′-Fam- AAAAGGTGGGCCTGAGGTTCA-3′.
The 6-Fam-AR1 and the TET-AR1 were used for mock and HpaII-digested samples, respectively.
The Sau96I-digested fluorescently labeled PCR products were analyzed by capillary electrophoresis, and the following primers were used: EGFR -Ex21-FWD1, 5′- CCTCACAGCAGGGTCTTCTCTGT-3′, and EGFR -Ex21-REV1, 5′-Fam- TCAGGAAAATGCTGGCTGACCTA-3′.