Words similar to fam-
Example sentences for: fam-
How can you use “fam-” in a sentence? Here are some example sentences to help you improve your vocabulary:
The primers and probes used in the real-time PCR were the following: Sense-Resistin, 5′- AGCCATCAATGATAGGATCCA-3′; Antisense-Resistin, 5′- TCCAGGCCAATGCTGCTTAT-3′; Resistin Probe, 5′-Fam- AGGTCGCCGGCTCCCTAATATTTAGGG-TAMRA-3′; Sense human 36B4 sense, 5′- TCGTGGAAGTGACATCGTCTTT-3′; Antisense 36B4, 5′- CTGTCTTCCCTGGGCATCA-3′; and 36B4 Probe, 5′-FAM- TGGCAATCCCTGACGCACCG-TAMRA-3′.
The final concentration of the AR1 primer was 3 pmol 6-Fam-AR1+7 pmol unlabelled AR1 and 10 pmol of unlabeled AR2 in a 50 μl reaction.
In 12 cases, exon 19 deletions were also studied by length analysis of fluorescently labeled PCR products on a capillary electrophoresis device, using the following primers: EGFR -Ex19-FWD1, 5′- GCACCATCTCACAATTGCCAGTTA-3′, and EGFR -Ex19-REV1, 5′-Fam- AAAAGGTGGGCCTGAGGTTCA-3′.
A specific exon 20 mutation (T790M) was also detected by length analysis of fluorescently labeled (FAM) PCR products on a capillary electrophoresis device (ABI 3100 Avant, Applied Biosystems, Foster City, California, United States), based on a new NlaIII restriction site created by the T790M mutation (2369 C→T), using the following primers: EGFR Ex20F, 5′-FAM- CTCCCTCCAGGAAGCCTACGTGAT-3′ and EGFR Ex20R 5′- TTTGCGATCTGCACACACCA-3′.
P1 is an "Amplifluor™"-primer, with a 5' hairpin and loop structure with a fluorophore and quencher at the base of the hairpin arms [ 13 12 16 ] . The two allele-specific OCPs have different backbone sequences complementary either to a FAM- or TET- labeled P1 allowing discrimination of the polymorphic base.
Loading...