Words similar to fam

Example sentences for: fam

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For spectratyping, PCRs were performed as described above with the following VB14- and VB17-specific 5′ primers: VB14m (5′- ACCCAAGATACCTCATCACAG-3′) and VB17 (5′- GACAGGACCCAGGGCAAG-3′), followed by a run-off PCR with downstream VB-specific primers: VB14 (5′- GGGCTTAAGGCAGATCTACT-3′) and VB17m (5′- TTTCAGAAAGGAGATATAGCT-3′), and FAM6-labeled BC63 3′ primer.
Forward primers were synthesized with either a fluorescent FAM tag (D18S481 and D18S452) or a fluorescent TET tag (D18S59) on the 5' end.
A specific exon 20 mutation (T790M) was also detected by length analysis of fluorescently labeled (FAM) PCR products on a capillary electrophoresis device (ABI 3100 Avant, Applied Biosystems, Foster City, California, United States), based on a new NlaIII restriction site created by the T790M mutation (2369 C→T), using the following primers: EGFR Ex20F, 5′-FAM- CTCCCTCCAGGAAGCCTACGTGAT-3′ and EGFR Ex20R 5′- TTTGCGATCTGCACACACCA-3′.
The P1 primer was the same for the two OCPs and detection and specificity was achieved by two allele-specific Amplifluor P2s carrying either a FAM or a TET fluorophore.
ERCA P1 primers were labeled with FAM (if paired with the normal OCP) or 5-Cy3 (if paired with the mutant OCP) and were RP-HPLC purified.