Words similar to exonuclease
Example sentences for: exonuclease
How can you use “exonuclease” in a sentence? Here are some example sentences to help you improve your vocabulary:
The Stoffel fragment of Taq polymerase is a truncated portion of the enzyme which retains polymerase activity but lacks the 5'->3' exonuclease activity of Taq polymerase [ 14 ] . The Stoffel fragment does not cut at the lac operator as does Taq polymerase [ 3 ] . It might therefore be a good choice of polymerase for jumping PCR.
Of the four protein areas that bind to the DNA 3' of the abasic site, mutating F266 (molego 10) or W280 (middle of molego 11) decreases APE activity [ 33 ] . The F266 mutation is particularly interesting, as the mutants at this position had reduced substrate specificity and enhanced 3'-exonuclease activity.
Reactions were stopped at 1 min for matched OCP and 4 hours for mismatched OCP by digestion with 0.75 units/μl Exonuclease I and 1.25 units/μl T7 gene 6 exonuclease (Amersham Pharmacia Biotech, Inc, NJ) at 37°C for 1 h, except the zero time points.
C-terminal deletions for mapping the interactions using two-hybrid assays were constructed using the exonuclease III/mung bean nuclease method (C-terminal deletions, Exonuclease III/Mung Bean Nuclease Deletion kit, Cat#460, NEW ENGLAND BioLabs company).
This alignment could be extended to the enzymatic core of three characteristic Mg 2+-dependent endonucleases: the bovine DNAseI [ 29 ] and two apurinic/apyrimidinic (AP) DNA-repair endonucleases, i.e. the E. coli Exonuclease III [ 30 ] and the human HAP1 protein [ 31 ] . These nucleases share the same catalytic residues and form a similar four-layered α/β-sandwich motif.
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