Words similar to exonuclease

Example sentences for: exonuclease

How can you use “exonuclease” in a sentence? Here are some example sentences to help you improve your vocabulary:

After amplification, reactions were incubated at 37°C for 15 min with 0.5 Uμl -1exonuclease I (USB) and 0.5 Uμl -1shrimp alkaline phosphatase (Amersham Life Sciences).
The regions containing the mutations were amplified by PCR using primers and conditions previously described [ 7 ] and later modified [ 23 ] . PCR products were treated with 1 U exonuclease and 1 U shrimp alkaline phosphatase for 15 min at 37°C.
APEs also have RNase H, 3'-exonuclease, and 3'-phosphodiester activities that are particularly high in the bacterial members of the family [ 26 13 ] .
This alignment could be extended to the enzymatic core of three characteristic Mg 2+-dependent endonucleases: the bovine DNAseI [ 29 ] and two apurinic/apyrimidinic (AP) DNA-repair endonucleases, i.e. the E. coli Exonuclease III [ 30 ] and the human HAP1 protein [ 31 ] . These nucleases share the same catalytic residues and form a similar four-layered α/β-sandwich motif.
The endonuclease activity of DNase 1 is reduced many fold by integrating this loop from E. coli exonuclease III, but the mutant cleaves at abasic sites in DNA with low efficiency [ 29 ] . Thus additional residues in the APEs control specificity while still allowing a reasonable rate of phosphorolytic cleavage.