Words similar to et-
Example sentences for: et-
How can you use “et-” in a sentence? Here are some example sentences to help you improve your vocabulary:
Binding studies of recombinant RGS3-GST fusion protein to 35S-Met-Gα-proteins were performed as detailed [ 9 ] . Glutathione-coupled beads were used to select 35S-Gα proteins bound to RGS3-GST, and were then eluted for separation by SDS/PAGE [ 9 ] . The amount and molecular size of 35S-Gα protein were determined using a Phosphor imaging device (Molecular Dynamics).
In the presence of the EGFR, ligand activation of LPA and ET-1 receptors did not increase EGFR phosphorylation even though both the LPA and ET-1 receptors stimulated ERK activity by 10-fold over basal [ 27 ] . Specifically, key protein intermediates may be physically associated with endogenous RTKs in HEK-293 cells, and therefore, down-regulation of the EGFR may negatively affect ERK phosphorylation by opioids.
The two experimental groups received intrathecally 10 μl of a mixture containing 10 μM of thiorphan and 20 μM of kelatorphan (to delay the degradation of the peptide ligand) and 10 μM of dermenkephalin (Tyr-D-Met-Phe-His-Leu-Met-Asp-NH 2 , Sigma, a selective δ agonist ( 14, 34)), in 0.9% NaCl.
An RGS3-GST fusion protein was incubated with an 35S-Met-Gα-protein, and the amount of labeled Gα-protein was determined using glutathione-coupled beads to "pull down" the RGS3-GST/ 35S-Gα-protein complex that was then separated and quantified by SDS/PAGE (Fig.
, [ 27 ] reported that endogenously-expressed LPA and endothelin-1 (ET-1) receptors in SK-N-MC cells do not activate ERKs unless the EGFR is co-expressed, although each GPCR is functional as evidenced by their ability to increase intracellular [Ca 2+].
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