Words similar to ere-tata-luciferase

Example sentences for: ere-tata-luciferase

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Stat3C did not modify the sensitivity of AR for other hormone responsive elements, since there was no response on the AR transcriptional activity when we used an estrogen-specific responsive reporter plasmid, ERE-TATA-luciferase (Fig 4, bottom right).
Luciferase reporter plasmids containing specific responsive elements for AR, GR, and PR (GRE-TATA-luciferase) and ER (ERE-TATA-luciferase) have been described elsewhere [ 29 30 ] ; another androgen-responsive reporter contained the fragment -286/+28 of the rat Probasin (PB) promoter, and was obtained from Dr. R. Matusik, Vanderbilt University, TN [ 31 ] . An expression plasmid for wild-type hAR was kindly provided by Dr. C. Chang, University of Rochester, NY.
For GR and PR, we used the same reporter plasmid as for AR, ARE-TATA-luciferase, since it shows promiscuous response to all three SHRs [ 30 ] . For ER, we used ERE-TATA-luciferase reporter that is specific for estrogen.
To test whether Stat3 could alter the specificity of AR to other HREs, CV-1 cells were cotransfected with reporter plasmids responsive to androgen (ARE-TATA-luciferase) or to estrogen (ERE-TATA-luciferase), in the presence or absence of AR and constitutively active Stat3C (Fig.