Words similar to ere-tata-luciferase

Example sentences for: ere-tata-luciferase

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Luciferase reporter plasmids containing specific responsive elements for AR, GR, and PR (GRE-TATA-luciferase) and ER (ERE-TATA-luciferase) have been described elsewhere [ 29 30 ] ; another androgen-responsive reporter contained the fragment -286/+28 of the rat Probasin (PB) promoter, and was obtained from Dr. R. Matusik, Vanderbilt University, TN [ 31 ] . An expression plasmid for wild-type hAR was kindly provided by Dr. C. Chang, University of Rochester, NY.
Stat3C did not modify the sensitivity of AR for other hormone responsive elements, since there was no response on the AR transcriptional activity when we used an estrogen-specific responsive reporter plasmid, ERE-TATA-luciferase (Fig 4, bottom right).
For GR and PR, we used the same reporter plasmid as for AR, ARE-TATA-luciferase, since it shows promiscuous response to all three SHRs [ 30 ] . For ER, we used ERE-TATA-luciferase reporter that is specific for estrogen.
To test whether Stat3 could alter the specificity of AR to other HREs, CV-1 cells were cotransfected with reporter plasmids responsive to androgen (ARE-TATA-luciferase) or to estrogen (ERE-TATA-luciferase), in the presence or absence of AR and constitutively active Stat3C (Fig.