Words similar to epharose

Example sentences for: epharose

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Induction of the enzyme protein was carried out as described before for the wild type HIV-1RT [ 29 ] . The enzyme with the hexahistidine-tag was purified from bacterial lysates by immobilized metal affinity chromatography [ 33 ] , while non-hexahistidine-tagged enzyme was purified using the phosphocellulose and Q-Sepharose columns as described previously [ 24 ] .
After concanavalin A-Sepharose 4B and nickel affinity column chromatography, the ammonium sulfate precipitated sCD39 was collected and resuspended in about 50 μl of 40 mM Tris-HCl (pH7.
Rlk was immunoprecipitated with affinity purified anti-Rlk antibody for 3 hours at 4°C and immune complexes were recovered with Protein A-Sepharose, washed as described above, and boiled in SDS-sample buffer.
The complexes were recovered with Protein A-Sepharose (Sigma) and washed two times with ice cold NP40 wash buffer (1% NP40, PBS, 1 mM sodium orthovanadate) and analyzed by Western blotting using either anti-Rlk serum or anti-Fyn serum (1:1000).
GAGs/polyanions purification was performed as follows: concentrated CM (15 ml) was dialyzed against buffer Q (50 mM Tris-HCl, pH 8.0, 0.2 M NaCl, 6 M Urea, 0.5% CHAPS), filtered through a 0.4 micron membrane and its soluble components subjected to anion-exchange column chromatography using Q-Sepharose Fast Flow (1 ml bed volume).