Words similar to epharose
Example sentences for: epharose
How can you use “epharose” in a sentence? Here are some example sentences to help you improve your vocabulary:
The complexes were recovered with Protein A-Sepharose (Sigma) and washed two times with ice cold NP40 wash buffer (1% NP40, PBS, 1 mM sodium orthovanadate) and analyzed by Western blotting using either anti-Rlk serum or anti-Fyn serum (1:1000).
2.1) purified from Streptomyces hyalurolyticus from ICN Pharmaceuticals, Coomassie Brilliant Blue from BioRad, and Q-Sepharose from Pharmacia.
Rlk was immunoprecipitated with affinity purified anti-Rlk antibody for 3 hours at 4°C and immune complexes were recovered with Protein A-Sepharose, washed as described above, and boiled in SDS-sample buffer.
Q-Sepharose Fast Flow was purchased from Amersham Pharmacia Biotech.
GAGs/polyanions purification was performed as follows: concentrated CM (15 ml) was dialyzed against buffer Q (50 mM Tris-HCl, pH 8.0, 0.2 M NaCl, 6 M Urea, 0.5% CHAPS), filtered through a 0.4 micron membrane and its soluble components subjected to anion-exchange column chromatography using Q-Sepharose Fast Flow (1 ml bed volume).