Words similar to epharose
Example sentences for: epharose
How can you use “epharose” in a sentence? Here are some example sentences to help you improve your vocabulary:
The complexes were recovered with Protein A-Sepharose (Sigma) and washed two times with ice cold NP40 wash buffer (1% NP40, PBS, 1 mM sodium orthovanadate) and analyzed by Western blotting using either anti-Rlk serum or anti-Fyn serum (1:1000).
Q-Sepharose Fast Flow was purchased from Amersham Pharmacia Biotech.
For immunoprecipitation, the lysates were incubated for 4 h at 4°C with 1 μg of antibody (all from Santa Cruz) pre-adsorbed on Protein A-Sepharose beads (Pharmacia).
2.1) purified from Streptomyces hyalurolyticus from ICN Pharmaceuticals, Coomassie Brilliant Blue from BioRad, and Q-Sepharose from Pharmacia.
Induction of the enzyme protein was carried out as described before for the wild type HIV-1RT [ 29 ] . The enzyme with the hexahistidine-tag was purified from bacterial lysates by immobilized metal affinity chromatography [ 33 ] , while non-hexahistidine-tagged enzyme was purified using the phosphocellulose and Q-Sepharose columns as described previously [ 24 ] .
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