Words similar to end-labeled

Example sentences for: end-labeled

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Purified TOP1 was pre-incubated with GST or GST-BTBD2 for 20 min before addition of the DNA substrate, which was either an end-labeled 36 bp fragment containing a single strong TOP1 cleavage site [ 24 ] , or a 161 bp PvuII-HindIII fragment of pBluescript (Stratagene) [ 25 ] ). End labeling of the DNA substrates was as previously described [ 24 25 ] . Cleavage reactions were continued for 20 min before stopping with 0.5% SDS.
This assay measures the ability of TOP1 to cleave end-labeled DNA substrates in the presence of the anticancer drug camptothecin, which blocks the ligation step of the cleavage /religation reaction of TOP1.
These were then probed with radioactive DNA oligonucleotide probes end-labeled with the StarFire system (Integrated DNA Technologies, Coralville, IA).
1are the background-subtracted images of the array after hybridization with end-labeled oligo(dT), performed in order to measure accessible PolyA in the spots.
The end-labeled GFP fusions and two of the transposon insertions, α s -GFP(18-20) and α s -GFP(92-94), showed clear localization to the plasma membrane (figure 4A).