Words similar to end-labeled

Example sentences for: end-labeled

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Electrophoretic mobility shift assays (EMSAs) were performed using a double stranded [ 32P]-end-labeled oligonucleotide covering the consensus E-box/HLH element (underlined) and flanking sequences from the RIIβ promoter (-305, 5'-GATCGCCGGGTTGCCATGGTTT CCGGGGAT CACGTGGGCGCGCGG-3', -268).
The gel mobility shift assay was used to compare the binding affinities of Top67 and the ZD domain to a 5' end-labeled single-stranded oligonucleotide 35 base in length.
1 ng) of 32P end-labeled consensus NF-κB oligonucleotide (5'-AGTTGAGGGGACTTTCCCAGGC-3') probe contained in the Promega gel shift assay system for 20-30 min at room temperature in 10 μl reaction volume using the kit provided reaction buffer.
an appropriate nested primer is 5'- 32P-labeled as previously described [ 54 ] ; 5 μl of the P1+7A reaction or the step-in verification reaction is denatured and annealed with 1 pmol of the end-labeled display primer and 0.5 units of Taq polymerase (Qiagen) in 10 μl final volume of 1× "Taq salts" (Qiagen).
A standard polymerase chain reaction (PCR) containing gamma [ 32P] ATP end-labeled forward primer, analysis of PCR products on denaturing polyacrylamide sequencing gels, and scoring of LOH on autoradiograms was performed as previously described [ 25 26 ] . All autoradiograms were visually analyzed.