Example sentences for: end-labeled

How can you use “end-labeled” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • This assay measures the ability of TOP1 to cleave end-labeled DNA substrates in the presence of the anticancer drug camptothecin, which blocks the ligation step of the cleavage /religation reaction of TOP1.

  • Total RNA (20 μg) was isolated from various cells and ran on a 1% formaldehyde-agarose gel overnight at 75 V, transferred onto a 0.2 μm nitrocellulose membrane (Millipore Inc.), UV cross-linked, and hybridized overnight at 42°C with 32P-end-labeled 40 mer oligo probes including p21/Waf1, C-myc, Pro-thymosin, Actin, Tat, and Ubiquitin (Loftstrand, Gaithersburg, Md.).

  • The gel mobility shift assay was used to compare the binding affinities of Top67 and the ZD domain to a 5' end-labeled single-stranded oligonucleotide 35 base in length.

  • The amplimers were UV cross-linked and hybridized with Rapid-Hyb buffer (Amersham) at 42°C using γ- 32P ATP end-labeled oligonucleotides (Table 1, G3PDH probe from Clontech) as probes.

  • 1are the background-subtracted images of the array after hybridization with end-labeled oligo(dT), performed in order to measure accessible PolyA in the spots.


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