Example sentences for: end-labeled

How can you use “end-labeled” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • 1and 2, in order to see whether we would obtain the same hybridization results using only end-labeled oligo(dT) as a probe, thereby providing evidence whether the polyA effect caused the two muscle cDNA hybridizations to produce similar results.

  • GFP was added to the N- or C-terminus of α s to create end-labeled constructs for comparison with the transposed GFP tribrid fusion proteins.

  • an appropriate nested primer is 5'- 32P-labeled as previously described [ 54 ] ; 5 μl of the P1+7A reaction or the step-in verification reaction is denatured and annealed with 1 pmol of the end-labeled display primer and 0.5 units of Taq polymerase (Qiagen) in 10 μl final volume of 1× "Taq salts" (Qiagen).

  • 1 ng) of 32P end-labeled consensus NF-κB oligonucleotide (5'-AGTTGAGGGGACTTTCCCAGGC-3') probe contained in the Promega gel shift assay system for 20-30 min at room temperature in 10 μl reaction volume using the kit provided reaction buffer.

  • Polymerase chain reaction (PCR) products (2 μ l) generated from either genomic DNA or cDNA were amplified using 5 pmol of nested sense V H family-specific FR3 primer and radiolabeled antisense nested J H consensus primer that had been end-labeled with γ 32-P (New England Nuclear, Beverly, MA, USA) using T4 polynucleotide kinase (Promega, Madison, WI, USA).


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