Example sentences for: end-labeled

How can you use “end-labeled” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • The 32P end-labeled oligonucleide probe (5 nM) was incubated with immunoprecipitation-purified FLAG-rDEK in a range of concentrations from one tenth to 10 times the estimated K d[app] (apparent dissociation constant) as described elsewhere [ 31 ] . Protein-bound DNA was separated from free probe as for EMSA in 1 × TBE (Tris-borate-EDTA buffer: Tris 89 mM, borate 89 mM, EDTA 2 mM).

  • The gel mobility shift assay was used to compare the binding affinities of Top67 and the ZD domain to a 5' end-labeled single-stranded oligonucleotide 35 base in length.

  • Electrophoretic mobility shift assays (EMSAs) were performed using a double stranded [ 32P]-end-labeled oligonucleotide covering the consensus E-box/HLH element (underlined) and flanking sequences from the RIIβ promoter (-305, 5'-GATCGCCGGGTTGCCATGGTTT CCGGGGAT CACGTGGGCGCGCGG-3', -268).

  • This assay measures the ability of TOP1 to cleave end-labeled DNA substrates in the presence of the anticancer drug camptothecin, which blocks the ligation step of the cleavage /religation reaction of TOP1.

  • 1and 2, in order to see whether we would obtain the same hybridization results using only end-labeled oligo(dT) as a probe, thereby providing evidence whether the polyA effect caused the two muscle cDNA hybridizations to produce similar results.


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