Words similar to end-labeled

Example sentences for: end-labeled

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A standard polymerase chain reaction (PCR) containing gamma [ 32P] ATP end-labeled forward primer, analysis of PCR products on denaturing polyacrylamide sequencing gels, and scoring of LOH on autoradiograms was performed as previously described [ 25 26 ] . All autoradiograms were visually analyzed.
The 32P end-labeled oligonucleide probe (5 nM) was incubated with immunoprecipitation-purified FLAG-rDEK in a range of concentrations from one tenth to 10 times the estimated K d[app] (apparent dissociation constant) as described elsewhere [ 31 ] . Protein-bound DNA was separated from free probe as for EMSA in 1 × TBE (Tris-borate-EDTA buffer: Tris 89 mM, borate 89 mM, EDTA 2 mM).
The end-labeled GFP fusions and two of the transposon insertions, α s -GFP(18-20) and α s -GFP(92-94), showed clear localization to the plasma membrane (figure 4A).
These were then probed with radioactive DNA oligonucleotide probes end-labeled with the StarFire system (Integrated DNA Technologies, Coralville, IA).
GFP was added to the N- or C-terminus of α s to create end-labeled constructs for comparison with the transposed GFP tribrid fusion proteins.