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Example sentences for: emsas

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Electrophoretic mobility shift assays (EMSAs)
Electrophoretic mobility shift assays (EMSAs) were performed using a double stranded [ 32P]-end-labeled oligonucleotide covering the consensus E-box/HLH element (underlined) and flanking sequences from the RIIβ promoter (-305, 5'-GATCGCCGGGTTGCCATGGTTT CCGGGGAT CACGTGGGCGCGCGG-3', -268).
EMSAs were carried out as previously described [ 30 ] , using 0.1-0.
1) first led us to investigate whether DEK in nuclear extracts binds to MHC class II Y-box regulatory elements in vitro . With a double-stranded oligonucleotide probe containing the HLA-DQA1*0101 Y-box sequence, EMSAs revealed similar binding activity in nuclear extracts from B lymphoid (Namalwa), T lymphoid (Jurkat, CEM), monocytoid (U937), and HeLa cell lines (Fig.
Competition EMSAs demonstrated sequence-specificity of the DQA1*0101 Y-box binding activity (Fig.