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Example sentences for: emsa

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Measurement of apparent dissociation constants using EMSA
EMSA: electrophoretic mobility shift assay
C; catalytic subunit, cAMP; cyclic adenosine monophosphate, CAT; chloramphenicol acetyl transferase, C/EBP; CAAT/enhancer binding protein, ChoRE; carbohydrate response element, CMV; cytalomega virus, 8-CPT-cAMP; 8-(4-chlorophenyl)thio-cAMP, CREB; cAMP-response element binding protein, EMSA; electrophoretic mobility shift assay, FAS; fatty acid synthase, FSH; follicle stimulating hormone, GIRE; glucose/insulin response element, hCG; human chorionic gonadotrophin, HLH; helix-loop-helix, IRE; insulin response element, MHC; major histocompability complex, PGHS; prostaglandin G/H synthase, PKA; cAMP-dependent protein kinase, R; regulatory subunit, USF; upstream stimulating factor, WB; Western blot.
In EMSA, the gel mobility of DNA-protein complexes depends on the overall mass of the binding proteins [ 38 ] and on the possible protein induced bending angle of DNA [ 39 ] . Since a single fusion protein was used in each binding reaction, the slight variation in the gel mobility of the DNA-protein complexes may reflect that KRC has more than one target, or that the targets were located at different positions within the 25-mer DNA.
Electrophoretic mobility shift assays (EMSA) were performed essentially as described previously [ 39 ] . Briefly, nuclear protein extracts (7 μg protein) prepared form A549 cells by the method of Osborn et al [ 40 ] were incubated with 5 × 10 5cpm (~0.