Words similar to emsa

Example sentences for: emsa

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Electrophoretic mobility shift assays (EMSA) were performed essentially as described previously [ 39 ] . Briefly, nuclear protein extracts (7 μg protein) prepared form A549 cells by the method of Osborn et al [ 40 ] were incubated with 5 × 10 5cpm (~0.
Moreover, EMSA demonstrated the presence of putative ARE on RAR α promoter, suggesting that AR (protein)-DNA interaction might be involved in the effects of T on RAR genes.
We then performed electrophoretic mobility shift assays (EMSA) using radiolabelled Ets oligonucleotide with nuclear extracts prepared from pEVRF-ets-1 transfected M12 cells (figure 3a, lane 3-9).
The 32P end-labeled oligonucleide probe (5 nM) was incubated with immunoprecipitation-purified FLAG-rDEK in a range of concentrations from one tenth to 10 times the estimated K d[app] (apparent dissociation constant) as described elsewhere [ 31 ] . Protein-bound DNA was separated from free probe as for EMSA in 1 × TBE (Tris-borate-EDTA buffer: Tris 89 mM, borate 89 mM, EDTA 2 mM).
At least three specific protein:DNA complexes are formed in EMSA using the CD5X oligonucleotides.