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Example sentences for: elisas

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Enzyme-linked immunosorbent assays (ELISAs) for MCP-1, IL-8, MIP-1β, and RANTES were purchased from R&D Systems, and transwell membranes (5 μm pore size in 24 wells) were purchased from Costar (Cambridge, MA).
For the TNF-β surrogate ELISAs, each well of a MaxiSorp Immuno plate (Nunc) is coated with 50 μl of target (1 μg/ml) overnight at 4°C.
All ELISAs were performed using the parent enzyme, BPN' Y217L, as a plate coating reagent.
In an attempt to understand the significance of the distributions of β-tubulin classes II and I + IV in normal and tumor breast tissues, we used ELISAs to quantify the Her2/neu protein levels in these tissues.
The mRNA ELISAs are performed in a similar manner except streptavidin-coated microtiter plates are blocked with PBS containing 2% non fat milk for 1 hr at room temperature, treated with RNaseZap, then coated with biotinylated RNA target (100 ng/well) by incubation for 1 hr at room temperature.