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Example sentences for: elisas

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For the TNF-β surrogate ELISAs, each well of a MaxiSorp Immuno plate (Nunc) is coated with 50 μl of target (1 μg/ml) overnight at 4°C.
All ELISAs were performed using the parent enzyme, BPN' Y217L, as a plate coating reagent.
The ELISAs were developed with ABTS in citrate buffer with 0.03% H2O2, at room temperature for 40 minutes and then read at OD = 405 nm.
T-cell proliferation was assessed on day 5 by [ 3H]thymidine incorporation [ 14 22 ] . Antigen (proteoglycan)-specific interferon-γ, IL-10, and IL-4 production by T cells was determined in media collected on day 4 using capture enzyme-linked immunosorbent assays (ELISAs) from BioSource International, Inc. (Camarillo, CA, USA).
The mRNA ELISAs are performed in a similar manner except streptavidin-coated microtiter plates are blocked with PBS containing 2% non fat milk for 1 hr at room temperature, treated with RNaseZap, then coated with biotinylated RNA target (100 ng/well) by incubation for 1 hr at room temperature.