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Example sentences for: elisas

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The mRNA ELISAs are performed in a similar manner except streptavidin-coated microtiter plates are blocked with PBS containing 2% non fat milk for 1 hr at room temperature, treated with RNaseZap, then coated with biotinylated RNA target (100 ng/well) by incubation for 1 hr at room temperature.
All ELISAs were performed using the parent enzyme, BPN' Y217L, as a plate coating reagent.
The method is adapted from standard protocols for indirect ELISAs [ 44 ] . The indirect ELISA involves the titration of antibodies reacted with tubulin in solution.
ELISAs were developed using ABTS in citrate buffer, 0.03% H2O2.
Competition ELISAs were performed using TNFα and TNFβ vs. the four phage clones at their ED 50 values (Figure 1a,1b,1c,1d).