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Example sentences for: elisa

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For cytokines assays, 1 × 10 6transduced cells were incubated on anti-CD3 coated plates for 72 h at 37°C/5% CO 2 . Culture supernatants were then harvested and an ELISA assay was performed to measure the production of IL-4, IL-10 and IFN-γ as previously described [ 30 ] . To assay proliferation, the transduced cells were plated in duplicate in microtiter plates (5 × 10 5/well) at a ratio of 1:5 with irradiated BALB/c splenocytes and the indicated doses of OVA peptide at 37°C/5% CO 2 . After 72 h, 50 μCi/ml [ 3H]thymidine (Amersham, Piscataway, NJ) was added to the well.
Jason DeVoss performed some of the ELISA immunoassays for antibodies.
The sandwich ELISA for gp41 six-helix bundles was performed as described [ 37 45 ] . HIV-1 IIIB and BaL virus preparations, respectively, treated with distinct anionic polymers, were precipitated with 3% polyethylene glycol 6000 (PEG) in order to separate virus particles from excess compounds.
Absolute measures of PGE-2 differed between assays that prevented the pooling of results; relative values may reflect differences in sample handling or assay conditions, or differences in ELISA kits themselves.
A human TNF ELISA kit (R&D Systems, Minneapolis, MN, USA) was used to determine the concentration of TNF in plasma from peripheral blood, in accordance with the manufacturer's instructions.