Example sentences for: electrophoretically

How can you use “electrophoretically” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • Proteins were separated by SDS-PAGE through a 12% gel, and then proteins were transferred electrophoretically to Immobilon P membrane.

  • Intermediate filament extracts of the cell lines [ 43 44 ] were electrophoretically blotted and detected with polyclonal anti GFAP (DAKO, Carpenteria, CA) and secondary reagents as above.

  • Samples of known numbers of cells from the cell lines and recombinant, purified Tag prepared from a baculovirus lysate [ 40 ] were loaded on 10% polyacrylamide discontinuous mini-gels (Bio-Rad, Hercules, CA) and electrophoresed conventionally [ 41 ] . Gels were then electrophoretically blotted [ 42 ] onto PVDF membrane (Bio-Rad) without methanol for 15 min at 100 V. Tag was visualized by immunostaining with PAb416 and alkaline phosphatase conjugated secondary antibody (Sigma) using chemiluminescent detection with CDP-Star substrate according to the manufacturers directions (Tropix, Bedford, MA).

  • Resulting samples were electrophoretically separated on a 1% Agarose (Kodak) gel and compared to a quantitative marker, BioMarker-EXT (BioVentures), to estimate the DNA concentration of each sample.

  • Samples were resolved by SDS-PAGE (9% polyacrylamide) and electrophoretically transferred to nitrocellulose filters (BioRad).


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