Words similar to electrophoretically
Example sentences for: electrophoretically
How can you use “electrophoretically” in a sentence? Here are some example sentences to help you improve your vocabulary:
Proteins were separated by SDS-PAGE through a 12% gel, and then proteins were transferred electrophoretically to Immobilon P membrane.
Intermediate filament extracts of the cell lines [ 43 44 ] were electrophoretically blotted and detected with polyclonal anti GFAP (DAKO, Carpenteria, CA) and secondary reagents as above.
Samples of known numbers of cells from the cell lines and recombinant, purified Tag prepared from a baculovirus lysate [ 40 ] were loaded on 10% polyacrylamide discontinuous mini-gels (Bio-Rad, Hercules, CA) and electrophoresed conventionally [ 41 ] . Gels were then electrophoretically blotted [ 42 ] onto PVDF membrane (Bio-Rad) without methanol for 15 min at 100 V. Tag was visualized by immunostaining with PAb416 and alkaline phosphatase conjugated secondary antibody (Sigma) using chemiluminescent detection with CDP-Star substrate according to the manufacturers directions (Tropix, Bedford, MA).
Resulting samples were electrophoretically separated on a 1% Agarose (Kodak) gel and compared to a quantitative marker, BioMarker-EXT (BioVentures), to estimate the DNA concentration of each sample.
Samples were resolved by SDS-PAGE (9% polyacrylamide) and electrophoretically transferred to nitrocellulose filters (BioRad).
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