Example sentences for: electrophoresed

How can you use “electrophoresed” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • After the final round of cRNA synthesis, aliquots of biotinylated cRNA from each sample were electrophoresed on 1% agarose gels in TBE buffer to check them for quality and length, the buffer and gel both contained a 500 ng/ml concentration of ethidium bromide.

  • PCR products were electrophoresed on a 2% agarose gel containing ethidium bromide.

  • 5 μl of the 50 ml reaction volume was electrophoresed on 0.8% agarose gels prior to Southern blotting and hybridization with a specific probe.

  • Fifty μg protein samples were heated in SDS-PAGE sample buffer containing β-mercaptoethanol and then electrophoresed on a 10% SDS-polyacrylamide gel.

  • Samples of known numbers of cells from the cell lines and recombinant, purified Tag prepared from a baculovirus lysate [ 40 ] were loaded on 10% polyacrylamide discontinuous mini-gels (Bio-Rad, Hercules, CA) and electrophoresed conventionally [ 41 ] . Gels were then electrophoretically blotted [ 42 ] onto PVDF membrane (Bio-Rad) without methanol for 15 min at 100 V. Tag was visualized by immunostaining with PAb416 and alkaline phosphatase conjugated secondary antibody (Sigma) using chemiluminescent detection with CDP-Star substrate according to the manufacturers directions (Tropix, Bedford, MA).


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