Example sentences for: electroblotting

How can you use “electroblotting” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • The electrophoresed samples were transferred onto ImmobilonP membranes (Millipore) by electroblotting for 1 hr in buffer containing 25 mM Bicine, 25 mM Bis-Tris, 1 mM EDTA, 10% methanol and 0.0025% Na dodecyl SO 4 . Prestained molecular mass markers (161-0372, BioRad) were supplemented with axonemal dynein from cilia of Tetrahymena (MM 528 kDa) in order to obtain a standard of sufficiently high molecular mass.

  • Proteins were transferred on to nitrocellulose membrane using an electroblotting apparatus (Bio-Rad, Richmond, CA).

  • Blots were prepared by electrophoresing 40 mg RNA from each time point per lane on 15% acrylamide gels, followed by electroblotting to ZetaProbe GT membranes (BioRad).

  • The whole cell extracts were separated on the 7.5% SDS PAGE and transferred onto nitrocellulose membrane by electroblotting.

  • For in vitro protein-protein interaction assays, 5 μg of either GST-CAF1 or GST purified proteins (prepared as described in [ 25 ] ) were subjected to 10% SDS/PAGE and transferred onto a polyvinylidene difluoride membrane (Millipore) by electroblotting.


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