Example sentences for: electroblotted

How can you use “electroblotted” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • The proteins were then electroblotted to nitrocellulose membranes at 125 mA for 45 min.

  • Proteins (5-20 μg/lane) were separated by using 10-20% acrylamide gels, and electroblotted onto nitrocellulose membranes.

  • Aliquots of 10 μg of protein were separated by SDS-PAGE and electroblotted to nitrocellulose.

  • Samples containing equivalent amounts of protein were resolved by 12% polyacrylamide SDS PAGE under reducing conditions and then electroblotted onto PVDF membranes.

  • Proteins were electroblotted to Hybond-P PVDF membranes (Amersham Pharmacia Biotech), blocked with gelatin, and probed with affinity purified rabbit anti-human myocilin antibody 129 (AB129; generated to human myocilin amino acids 156-171) [ 12 17 ] and an anti-rabbit IgG secondary antibody (Amersham).


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