Words similar to eif-

Example sentences for: eif-

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Lastly, elevated phosphorylation of eIF-2α has been used as a diagnostic marker of IFN response [ 30 ] . We, therefore, examined the phosphorylation status of eIF-2α in A549 cells following transfection with these dsRNAs.
The eIF-2 was precipitated with a polyclonal antibody against human eIF-2α (C-20) (Santa Cruz Biotech) in the presence of protein G-Sepharose (Amersham) for 1 h at 10°C, and then processed as described previously [ 21 ] . The immunoprecipitates were subjected to SDS-PAGE followed by autoradiography to examine eIF-2 phosphorylation.
Phosphorylated eIF2α competitively inhibits the guanine nucleotide exchange factor eIF-2B, which is responsible for the recycling of eIF2 by catalyzing the conversion of eIF2-GDP to the active form eIF2-GTP [ 36 ] . Thus the overall initiation of translation is inhibited when eIF2α becomes phosphorylated.
In positive controls, A549 cell treated with calcium mobilizers (thapsigargin or A23178) did increase eIF-2 phosphorylation, as has been shown earlier [ 30 ] . These results provide the first direct evidence that the 21-nt long double-stranded siRNAs fail to trigger interferon response in mammalian cells, and hence, can be used as specific antiviral agents.
To monitor total eIF-2α in the cell, a parallel A549 monolayer was treated identically except that the labeled orthophosphate was omitted.