Words similar to edta-free

Example sentences for: edta-free

How can you use “edta-free” in a sentence? Here are some example sentences to help you improve your vocabulary:

After 24 h, the cells were harvested in PBS, collected by centrifugation, and lysed in 5 volumes of 40 mM Tris-HCl, pH 7.4, containing complete mini-EDTA-free protease inhibitor cocktail (Sigma, St. Louis, MO).
After 48 hours cells were harvested by scraping in PBS, centrifuged at 1,000 × g for 10 minutes, and gently resuspended in around 10 ml of a 50 mM Tris pH 7.5, 250 mM sucrose buffer containing an EDTA-free protease inhibitor cocktail (Roche).
After 2 hours of incubation at 37°C, cells were washed one time with PBS and subsequently lysed in 0.5 ml of lysis/wash buffer containing 1% NP40 in Tris-buffered saline plus an EDTA-free protease inhibitor cocktail (Roche; Indianapolis, USA) for 20 minutes at 4°C.
5 μg total protein) were incubated on the plates with various concentrations of the [ 3H]-Hh-Ag 1.5 (plus and minus competitors) in binding buffer plus EDTA-free protease inhibitor cocktail (Roche) at a final volume of 1 ml for approximately 45 hours at 37°C to allow binding to reach apparent equilibrium.
5 μg total protein) were incubated in polypropylene tubes with 2 nM [ 3H]-Hh-Ag 1.5 in the presence or absence of 2 μM competitor in binding buffer (50 mM Tris pH 7.5, 5 mM MgCl, 1 mM EDTA, 0.1 % bovine serum albumin) plus EDTA-free protease inhibitor cocktail (Roche) in a final volume of 250 μl for 1-26 hours at 37°C.