Words similar to edta

Example sentences for: edta

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For experiments in Figures 4Cand 6A, immunoprecipitation of RENT through Myc9-Net1 was carried out as described earlier [ 8 ] , except that EDTA (final 2 mM) was included in the lysis buffer.
To obtain nuclei, (4-6) × 10 7cells of each cell line grown in the presence and absence of 2.5 μM SAHA were washed twice in PBS, followed by suspension in 0.3 ml nuclear buffer consisting of 2 mM MgCl 2 , 5 mM K 2 HPO 4 , 0.1 mM EDTA, 1 mM PMSF, 20 μg/ml aprotinin, 20 μg/ml leupeptin, 0.1 mM Na 3 VO 4 and 5 mM β-glycerophosphate.
The column was washed with 5 column volumes of Buffer A + 150 mM NaCl and 5 column volumes of Buffer A + 250 mM NaCl (no EDTA).
Cry1Aa binding ability was also checked by slot blotting fractions to PVDF membrane and probing with biotinylated Cry1Aa [ 26 ] . Fractions with Cry1Aa-binding ability and APN enzymatic activity were concentrated to 2 ml volumes and loaded on a Superdex 200 size-exclusion column (120 ml bed volume) using Hepes-buffered saline (HBS; 10 mM Hepes, 150 mM NaCl, 3.4 mM EDTA, pH 7.4) as running buffer.
p35-ACS interaction is sensitive to high salt, temperature and EDTA