Words similar to edta
Example sentences for: edta
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100 micrograms of protein) with 220 μl of incubation buffer (140 mM KCl, 0.3 mM EDTA, 5 mM MgCl 2 , 10 mM Mes, pH 6.5) containing labeled thiamine or ThDP at various concentrations.
2/ 10 mM MgCl 2 / 0.2 mM EDTA/ 5 μM ATP/10 μCi 32P-ATP) in a final volume of 40 μl.
To terminate the reaction, 3 μl of the reaction mixture was withdrawn every three minutes and mixed with 7 μl of 10 mM EDTA in 50 mM Tris-HCl, pH 8.0 (this is an important modification of our previous protocol, as the EDTA stops GTP hydrolysis).
Cells were resuspended with 4 ml/g cells in 50 mM Tris pH 8, 1 mM DTT, 1 mM EDTA.
To assemble Cdc14 beads with Net1N for two assays (Figure 4B, detection method: Western blotting), 2 μl of rabbit-anti-GST antibodies were absorbed to 40 μl of protein A beads (Sigma) in 100 μl of HBS [20 mM HEPES (pH 7.2), 150 mM NaCl] + 2 mM EDTA + 0.1 mg/ml BSA + 1 mM DTT (HEBD) buffer for 1 hr.
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