Words similar to edta
Example sentences for: edta
How can you use “edta” in a sentence? Here are some example sentences to help you improve your vocabulary:
Hybridization was carried out with the 35S-labeled RNA probe (4-6 × 10 6cpm/ml) in a solution containing 50% (vol/vol) deionized formamide, 0.3 M NaCl, 10 mM Tris (pH 8.2), 1 mM EDTA, 0.05% yeast tRNA, 10 mM dithiothreitol, 1 × Denhardt's solution and 10% dextran sulfate.
9, 300 mM NaCl, 50 mM KCl, 0.1 mM EDTA, 10% glycerol, 1 mM DTT, and 1 mM PMSF).
Ice cold PBS was added to the cells after 2 minutes OKT3 treatment and the cells were quickly pelleted and lysed in 0.5 ml TENV (10 mM Tris 7.5, 150 mM NaCl, 5 mM EDTA, 1 mM Na 3 VO 4 ) containing 1% Triton X-100.
Next, the cells were resuspended at 3 × 10 6cells/ml in ice cold sonication buffer (250 mM sucrose; 10 mM HEPES, pH 7.4; 1 mM EDTA; 5 mM MgCl 2 ; 1 mM DTT; 0.1 mM GTP; 1 μg/ml leupeptin, 1 μg/ml pepstatin A, and 1 μg/ml aprotinin; 1 mM PMSF) and sonicated for 10 seconds.
MTT assays were performed as follows [ 14]: MCF7/WT and MCF7/ADR cells were each harvested with 0.05% trypsin/EDTA and counted.
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