Example sentences for: ecl

How can you use “ecl” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • COX-1 and COX-2 antibodies (Santa Cruz Biotechnology Inc) were used in combination with standard ECL techniques.

  • The subunits were visualized using SuperSignal ECL reagent (Pierce, Rockford, IL).

  • One quarter of each fraction was subjected to SDS-PAGE and immunoblot analysis using the 9E10 monoclonal antibody against the myc epitope (Oncogene Research Products, Cambridge, MA), followed by HRP-conjugated goat anti-mouse IgG and ECL detection reagent (Amersham Corp, Arlington Hts.

  • The membranes were probed with rat calbindin antibody (1:50,000 obtained from Dr. Anthony Iacopino, Marquette University, USA) or human COX-2 antibody (1:3,000) (obtained from Dr. Daniel Simmons, BYU, USA) and detected by the enhanced chemiluminescence (ECL) Western blotting system (Amersham, Arlington Height, IL, USA).

  • The blots were blocked in 5% fat-free milk in PBS-Tween at room temperature for 1 hour, then exposed to primary antibodies (mouse anti-human MMP-1, MMP-2, MMP-3 or MMP-9 antibodies; Calbiochem, Cambridge, MA, USA), and subsequently detected using HRP conjugated rabbit anti-mouse IgG (ICN Biomedical, Costa Mesa, CA, USA) in conjunction with an enhanced chemiluminescence detection system (ECL, Amersham Pharmacia Biotech, Little Chalfont, Buckinghamshire, England).


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