Example sentences for: ecl

How can you use “ecl” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • The primary antibody was incubated in the 5% non-fat milk with the proteins on the membrane overnight at 4°C, and the protein of interest was visualized by the enhanced chemiluminescent method (ECL).

  • Lastly, membranes were washed 3 times and developed using the enhanced chemiluminescence (ECL) detection system (Amersham, La Jolla, CA).

  • The membranes were washed 3 times for 10 min each with TTBS, incubated in anti-mouse or anti-rabbit HRP (1:10,000) in 1% milk for 1 hr and washed as above, the bands visualized using the ECL (Amersham).

  • Blots were revealed using peroxidase conjugated goat anti-mouse IgG antibody and chemiluminescence with an ECL kit (Amersham).

  • 1% v/v Tween 20 (PBS-T) overnight at 4°C, then subjected to immunoblotting and ECL (Amersham, Arlington, IL) detection.


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