Words similar to ecl
Example sentences for: ecl
How can you use “ecl” in a sentence? Here are some example sentences to help you improve your vocabulary:
Gdn-HCl, guanidine-hydrochloride; HRP, horseradish peroxidase; ECL, enhanced chemiluminescence; PBS, phosphate-buffered saline; BSA, bovine serum albumin.
Bound antibodies were detected by a chemiluminescent detection system (ECL; Amersham Pharmacia Biotech, Piscataway, NJ) as recommended by the manufacturer's protocol.
4 for MRLC), blotted to PVDF or nitrocellulose membranes, immunostained with our rabbit polyclonal anti-HSP20 antibody (1:5000) or rabbit polyclonal anti-MRLC antibody (1:4000 in 1% bovine serum albumin and 0.01% sodium azide), and detected with enhanced chemifluorescence (ECF) for HSP20 and enhanced chemiluminescence (ECL) for MRLC [ 22 ] . A representative blot is shown in Fig.
MAdCAM-1 (mucosal addressin cell adhesion molecule-1), IL-10 (Interleukin-10), HEV (high endothelial venular), ECAMs (endothelial cell adhesion molecules), IBD (inflammatory bowel diseases), UC (ulcerative colitis), CD (Crohn's disease), ICAM-1 (intracellular adhesion molecule 1), VCAM-1 (vascular adhesion molecule 1), NF-kB (nuclear transfection factor kB), TNF-α (tumor necrosis factor alpha), GM-CSF (granulocyte-macrophage colony-stimulating factor), GCSF (granulocute colony stimulating factor), ECL (enhanced chemiluminescence)
Forty-eight hours after transfection, cells were lysed and membrane and supernatant fractions harvested as described previously [ 8 ] . 10 μg of membrane proteins and normalized volumes of the supernatants were resolved by SDS-polyacrylamide electrophoresis (10%), transferred to nitrocellulose, and probed with a monoclonal antibody to the EE epitope [ 34 ] . The antigen-antibody complexes were visualized with ECL chemiluminescence (Amersham Biosciences, Piscataway, NJ).
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