Words similar to ecl
Example sentences for: ecl
How can you use “ecl” in a sentence? Here are some example sentences to help you improve your vocabulary:
COX-1 and COX-2 antibodies (Santa Cruz Biotechnology Inc) were used in combination with standard ECL techniques.
The subunits were visualized using SuperSignal ECL reagent (Pierce, Rockford, IL).
One quarter of each fraction was subjected to SDS-PAGE and immunoblot analysis using the 9E10 monoclonal antibody against the myc epitope (Oncogene Research Products, Cambridge, MA), followed by HRP-conjugated goat anti-mouse IgG and ECL detection reagent (Amersham Corp, Arlington Hts.
The membranes were probed with rat calbindin antibody (1:50,000 obtained from Dr. Anthony Iacopino, Marquette University, USA) or human COX-2 antibody (1:3,000) (obtained from Dr. Daniel Simmons, BYU, USA) and detected by the enhanced chemiluminescence (ECL) Western blotting system (Amersham, Arlington Height, IL, USA).
The blots were blocked in 5% fat-free milk in PBS-Tween at room temperature for 1 hour, then exposed to primary antibodies (mouse anti-human MMP-1, MMP-2, MMP-3 or MMP-9 antibodies; Calbiochem, Cambridge, MA, USA), and subsequently detected using HRP conjugated rabbit anti-mouse IgG (ICN Biomedical, Costa Mesa, CA, USA) in conjunction with an enhanced chemiluminescence detection system (ECL, Amersham Pharmacia Biotech, Little Chalfont, Buckinghamshire, England).
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