Words similar to ecl
Example sentences for: ecl
How can you use “ecl” in a sentence? Here are some example sentences to help you improve your vocabulary:
The primary antibody was incubated in the 5% non-fat milk with the proteins on the membrane overnight at 4°C, and the protein of interest was visualized by the enhanced chemiluminescent method (ECL).
ECL reagents were from Amersham Pharmacia Biotech, Inc. (Piscataway, NJ).
Proteins were transblotted to Immobilon P membrane, blots were probed with antibody AP-101, and bound antibodies were detected by ECL.
Protein (250 μg) from the control-infected or HIV-1-infected podocytes on day 14 of the differentiation protocol was also analyzed by western blot for total pRb (ECL detection of a 1:1000 dilution of rabbit anti-Rb, Santa Cruz Biotechnology) and phospho-Rb (Ser780) (ECL detection of a 1:500 dilution of rabbit anti-phospho-Rb (Ser780) antibody binding, Cell Signaling).
After washing in TBST, the ERβ protein was visualized with a chemiluminescent detection system (ECL; Amersham Pharmacia Biotech, Piscataway, NJ) as recommended by the manufacturer, and exposed on Kodak XAR film (Eastman Kodak, Rochester, NY).