Example sentences for: ecl

How can you use “ecl” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • Nitrocellulose membranes (Hybond ECL), secondary antibodies, enhanced chemiluminescence (ECL) reagent, and film (Hyperfilm ECL) were obtained from Amersham Pharmacia Biotech (Piscataway, NJ).

  • Immunoreactive proteins were visualized using a horseradish peroxidase-sensitive ECL chemiluminescent Western blotting (WB) kit (Pierce).

  • Antibody binding was visualized by enhanced chemiluminescence (ECL, Amersham) according to manufacturers directions.

  • Fibrosarcoma PAI-1 and wtPAI-1 were transferred onto nitrocellulose paper and the proteins were revealed with anti-human PAI-1 monoclonal antibodies, followed by horseradish peroxidase (HRP) conjugated rabbit anti-mouse antibody, and enhanced chemiluminescence (ECL).

  • Bound antibodies were detected by a chemiluminescent detection system (ECL; Amersham Pharmacia Biotech, Piscataway, NJ) as recommended by the manufacturer's protocol . For exposure (first 5 min, and second up to 2 h) we used Kodak XAR film (Eastman Kodak, Rochester, NY).


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