Words similar to ecfp
Example sentences for: ecfp
How can you use “ecfp” in a sentence? Here are some example sentences to help you improve your vocabulary:
For whole mount photographs (Figures 2and 4), unfixed embryos were photographed using a Nikon epifluorescence microscope fitted with Chroma filter sets for ECFP (cyan GFP Ex436/20 Dm455 Bar480/40) and EYFP (yellow GFP Ex500/20 Dm515 Bar535/30).
This is not surprising, given the higher quantum yield and extinction coefficient of EYFP as compared to ECFP, both of which result in higher fluorescence intensity.
ECFP or EYFP was inserted into pBigT, and the resulting sequence was inserted into a vector containing ROSA26 genomic sequences to permit homologous recombination (Fig 1A).
Using dual EYFP/ECFP imaging, we were able to directly compare translocation of both constructs in the same cell.
Clones representing unique fluorescent fusion proteins were digested with Srf I to remove the Kan r selection cassette and re-ligated to generate full-length GluR1-EGFP/ECFP fusions.