Words similar to ecfp
Example sentences for: ecfp
How can you use “ecfp” in a sentence? Here are some example sentences to help you improve your vocabulary:
In 288 co-transfections, there were 20 wells with EGFP fluorescence, 21 wells with ECFP fluorescence, and 2 wells with both EGFP and ECFP fluorescence.
ECFP/EYFP fluorescence changes with respect to time for regions of interest corresponding to an area of Golgi membrane were determined by calculating the F t /F 0 , where F t is the background- and bleach-corrected ECFP or EYFP fluorescence at time = t and F 0 is the background-corrected ECFP or EYFP fluorescence at time = 0 s.
This is consistent with previous reports of Tn 5 preferentially inserting into particular locations in the target sequence [ 17 18 ] . Since these "hotspots" could become a limiting factor in the number of unique insertions recovered within a target sequence, the second reading frame through the Tn 5 MEs was used for the ECFP transposon.
Clones representing unique fluorescent fusion proteins were digested with Srf I to remove the Kan r selection cassette and re-ligated to generate full-length GluR1-EGFP/ECFP fusions.
In addition, the two different reading frames present in the MEs were used to create the two different transposons, and ECFP was used in place of EGFP in
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