Words similar to ecfp
Example sentences for: ecfp
How can you use “ecfp” in a sentence? Here are some example sentences to help you improve your vocabulary:
MDCK cells were co-transfected with EYFP-cPLA 2 and a cPLA 2 with a S505A mutation fused to ECFP (ECFP-cPLA 2 S505A).
We have constructed two reporter lines of mice that express EYFP or ECFP only in cells expressing the Cre recombinase, and their daughter cells, by targeting these cDNAs into the ubiquitously expressed ROSA26 locus, preceded by a loxP flanked "stop" sequence.
To make the R26R-CFP targeting construct pECFP was digested with AgeI, Klenow filled then digested with NotI to excise the ECFP cDNA.
ECFP/EYFP fluorescence changes with respect to time for regions of interest corresponding to an area of Golgi membrane were determined by calculating the F t /F 0 , where F t is the background- and bleach-corrected ECFP or EYFP fluorescence at time = t and F 0 is the background-corrected ECFP or EYFP fluorescence at time = 0 s.
The plasmids pEYFP-N1 and pECFP containing cDNA for EYFP and ECFP were purchased from Clontech Laboratories Inc.
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