Example sentences for: ecfp

How can you use “ecfp” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • For whole mount photographs (Figures 2and 4), unfixed embryos were photographed using a Nikon epifluorescence microscope fitted with Chroma filter sets for ECFP (cyan GFP Ex436/20 Dm455 Bar480/40) and EYFP (yellow GFP Ex500/20 Dm515 Bar535/30).

  • This is not surprising, given the higher quantum yield and extinction coefficient of EYFP as compared to ECFP, both of which result in higher fluorescence intensity.

  • ECFP or EYFP was inserted into pBigT, and the resulting sequence was inserted into a vector containing ROSA26 genomic sequences to permit homologous recombination (Fig 1A).

  • Using dual EYFP/ECFP imaging, we were able to directly compare translocation of both constructs in the same cell.

  • Clones representing unique fluorescent fusion proteins were digested with Srf I to remove the Kan r selection cassette and re-ligated to generate full-length GluR1-EGFP/ECFP fusions.


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