Example sentences for: ebp

How can you use “ebp” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • Binding reactions were carried out at room temperature for 30 min in a total volume of 15 μl and contained 2-5 μg of nuclear extracts, 5 μl of 5X gel shift binding buffer (20% glycerol, 5 mM MgCl 2 , 2.5 mM EDTA, 2.5 mM DTT, 250 mM NaCl, 50 mM Tris-HCl, pH 7.5), 2 μg poly (dI-dC) and 3 × 10 4cpm/μl of [ 32P] labeled CREB (5'-AGA GAT TGC CTG ACG TCA GAG AGC TAG-3'), NF-κB (5'-AGT TGA GGG GAC TTT CCC AGG C-3') (Promega Gel Shift Assay Systems, Madison, WI, USA) or CCAAT/enhancer binding protein (C/EBP) (5'-TGC AGA TTG CGC AAT CTG CA-3') (Santa Cruz Biotechnology, Inc., Santa Cruz, CA, USA) oligonucleotides.

  • Inhibiting the expression of eIF4E by expressing antisense RNA reverses ras-mediated transformation of cultured cells as indicated by decreased efficiency of growth in soft agar and tumor formation in nude mice [ 20 ] . Similarly, antisense-mediated reduction of eIF4E in breast cancer cells inhibits both their tumorigenic and angiogenic properties [ 21 ] . On the other hand, overexpression of 4EBP reverses transformation mediated by v-src [ 18 ] .

  • Administration of endotoxin to the animals also induced C/EBP nuclear binding activity.

  • Electrophoretic mobility shifts assays were done by mixing a radiolabeled oligonucleotide containing a consensus, high affinity C/EBP binding site (GATCGAGCCCCATTGCGCAATCATAGATC) together with extracts prepared from transfected cells as previously described [ 58 59 ] . Competition with the same, unlabeled oligonucleotide in 1, 10 or 100 molar excess of the radiolabeled probed indicated specific binding.

  • 4EBP phosphorylation is thought to be mediated by mammalian target of rapamycin (mTOR) in mitogen stimulated cells [ 9 10 11 ] .

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