Words similar to ebp
Example sentences for: ebp
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It is possible that NF-κB and CREB mediate the early phases of neutrophil activation, while C/EBP proteins function to maintain this response [ 50 ] . The observation that the temporal pattern of expression of activated C/EBP and PKB-α are similar suggests co-ordinated regulation of these proteins [ 51 ] . A decrease in activation of transcription factors like NF-κB and CREB and an increase in C/EBP may also contribute to reduced activity of pro-apoptotic genes, resulting in prolonged longevity of neutrophils in the lung vasculature.
Clinical trials using the rapamycin analog CCI-779 show promise for this strategy [ 22 ] . Rapamycin and its synthetic analogs block mTOR kinase activity leading to dephosphorylation of 4EBP and other mTOR substrates.
Binding reactions were carried out at room temperature for 30 min in a total volume of 15 μl and contained 2-5 μg of nuclear extracts, 5 μl of 5X gel shift binding buffer (20% glycerol, 5 mM MgCl 2 , 2.5 mM EDTA, 2.5 mM DTT, 250 mM NaCl, 50 mM Tris-HCl, pH 7.5), 2 μg poly (dI-dC) and 3 × 10 4cpm/μl of [ 32P] labeled CREB (5'-AGA GAT TGC CTG ACG TCA GAG AGC TAG-3'), NF-κB (5'-AGT TGA GGG GAC TTT CCC AGG C-3') (Promega Gel Shift Assay Systems, Madison, WI, USA) or CCAAT/enhancer binding protein (C/EBP) (5'-TGC AGA TTG CGC AAT CTG CA-3') (Santa Cruz Biotechnology, Inc., Santa Cruz, CA, USA) oligonucleotides.
The observed decline in cyclin D1 is expected since constitutively active 4EBP should reverse cyclin D1 expression mediated by eIF4E overexpression.
As observed with NF-κB, constitutive CREB and C/EBP nuclear binding activities were not evident in cells from control animals (Fig.
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