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Example sentences for: eberwine

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A widely used protocol, developed by Eberwine and colleagues, uses T7-based linear amplification [ 14 ] . In this procedure, double stranded cDNAs are synthesized from mRNA by reverse-transcription (priming off the 3' polyA tail) and second strand synthesis.
For first strand cDNA synthesis, 3 μg (unless otherwise specified) tumor total RNA, Universal Human Reference total RNA (Stratagene ®), or 150 ng poly(A) +RNA was mixed with 1 μg Eberwine primer (Operon ®) in RNase-free water to a total volume of 9 μl.
The reaction products are used as template for IVT using the Eberwine method [ 14 ] . From a starting material of ChIP DNA from ~2 × 10 8yeast cells this protocol yields between 30 and 60 μg RNA.
The amplification procedure we used is based on protocols described by Eberwine [ 21, 22, 24], Wang et al.
The most commonly used mechanism for RNA amplification is a T7 based linear amplification method first developed by Van Gelder, Eberwine and coworkers [ 19 20 21 ] . This method utilizes a synthetic oligo(dT) primer containing the phage T7 RNA polymerase promoter to prime synthesis of first strand cDNA by reverse transcription of the poly(A) +RNA component of total RNA.