Words similar to eagle
Example sentences for: eagle
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NIH 3T3 fibroblasts (from the American Type Culture Collection) and COS-7 cells (kindly provided by Dr. S. Hann, Vanderbilt University) were grown in Dulbecco's Modified Eagle Medium (DMEM) with 10% calf serum (Colorado Serum Company), 0.22% sodium bicarbonate, 4 mM L-glutamine, 25 U of penicillin G sodium per mL, and 25 mg of streptomycin per mL.
Trophoblast cells are usually separated and grown in media containing phenol red (PhR) [ 35 ] . Phenol red is a weak estrogen with apparent biological effects, and the ERα binding affinity in Dulbecco's Modified Eagle's Medium (DMEM) is equivalent to levels of E2 as high as 0.45 nM [ 36 ] . Although relatively low when compared to the E2 levels in pregnancy, this estrogenic binding affinity of PhR is close to that of E2 during the preovulatory peak [ 4 5 ] . Therefore, we included studies with PhR+, PhR-/E2- and PhR-/E2+ media in order to determine the effect of estrogens on differentiation of cultured trophoblast cells (syncytium formation) derived from normal and abnormal placental types.
For determination of basal gene-expression profiles without androgen stimulation, fibroblasts were cultured on 150-mm plastic dishes at 37°C with 5% CO 2 . To eliminate possible artifacts due to differing states of proliferation, cells were grown to confluence, at which point they enter G 0 arrest [ 31 ] . They were maintained in phenol-red-free DMEM F12 (Dulbecco's modified Eagle Medium with the nutrient mix F12; Gibco) containing L-glutamine, 15 mM Hepes buffer, penicillin/streptomycin (Gibco) and 12.
Book -- The First Eagle , by Tony Hillerman;
[From a play review in The Berkshire Eagle , . Submitted by .]
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