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Example sentences for: dslet

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In cells pretreated with DSLET (10 μM; 24 hours) to induce down-regulation of δ-ORs [ 21 ] , DSLET (1 μM) was no longer able to activate the ERK cascade, but the ERK response to acute EGF (10 ng/ml) was unchanged compared to medium-treated controls (Figure 6).
WT-δ-OR-expressing HEK-293 cells were exposed to increasing concentrations of either DSLET (1-1000 nM) or EGF (0.
Despite the lack of direct evidence that DSLET induces the transactivation of co-expressed RTKs, it is possible that the EGFR, in some way, contributes to ERK activation by opioids.
Moreover, we pre-treated C6-δ-OR cells with the IR antagonist, hydroxy-2-napthalenylmethylphosphonic acid-tris acetoxymethylester (HNMPA-(AM) 3 ; 100 μM), or the PDGFR antagonist, (tyrosphostin 9; 100 μM) before the addition of DSLET (1 μM).
After immunoprecipitation of the FLAG-tagged δ-OR or the EGFR with the appropriate antibodies, we found that only DSLET induced tyrosine phosphorylation of the δ-OR, whereas only EGF potentiated EGFR phosphorylation (Figure 4).