Words similar to dslet
Example sentences for: dslet
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As stated earlier, either DSLET or EGF increased the appearance of phosphorylated ERK in a time and concentration dependent manner.
Furthermore, immunoprecipitation of the δ-OR, followed by immunoblotting using anti-phosphotyrosine antibodies, revealed that these inhibitors did not attenuate tyrosine phosphorylation of δ-ORs after DSLET treatment (Figure 9).
Moreover, we pre-treated C6-δ-OR cells with the IR antagonist, hydroxy-2-napthalenylmethylphosphonic acid-tris acetoxymethylester (HNMPA-(AM) 3 ; 100 μM), or the PDGFR antagonist, (tyrosphostin 9; 100 μM) before the addition of DSLET (1 μM).
Despite the lack of direct evidence that DSLET induces the transactivation of co-expressed RTKs, it is possible that the EGFR, in some way, contributes to ERK activation by opioids.
In conclusion, we report that the δ-OR agonist, DSLET, induces significant ERK activation in the absence of any increase in phosphorylation of the co-expressed EGFR in HEK-293 cells.