Words similar to drg

Example sentences for: drg

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Twenty four genes detected by the arrays as being present in the DRG and displaying up-, down- or no regulation three days after sciatic nerve axotomy were analyzed by replicate quantitative slot blot (Figure 2) and Northern blot analysis (Figure 3).
Total RNA was extracted from homogenized DRG samples using acid phenol extraction (TRIzol reagent, Gibco-BRL).
Oligonucleotide microarrays provide the capacity to analyze parallel changes in many thousands of genes, and have been used successfully to examine expression profile changes in many neuronal and non-neuronal systems [ 7 ] including DRG neurons [ 8 9 ] . Studying the effect of peripheral nerve injury on gene expression profiles in the DRG offers several advantages.
Sections from the DRG were processed for the visualization of MIF and substance P as follows: sections were exposed to anti-MIF (1:200) and anti-substance P (guinea pig polyclonal; 1:1000; Chemicon; Tamecula, CA) overnight at 4°C.
The control human DRG sample was pooled from total RNA (n = 8; prepared by Clontech) obtained from post-mortem tissues.