Words similar to double-stranded
Example sentences for: double-stranded
How can you use “double-stranded” in a sentence? Here are some example sentences to help you improve your vocabulary:
Double-stranded cDNA product was purified via phenol-chloroform extraction, and then used as template in first round of in vitro transcription (IVT) of cRNA.
This result indicated that with regard to the mechanism of E. coli topoisomerase I, the ZD domain was likely to function as a single-stranded DNA binding domain instead of having double-stranded DNA binding function as previously suggested [ 21 ] . Even though Zn(II) binding transcription factors that recognise specific double-stranded DNA are well represented in eukaryotes [ 23 24 ] , there are also numerous examples of Zn(II) coordination being required for interaction with single-stranded nucleic acid or damaged DNA with single-strand characteristics [ 24 25 26 27 ] .
Approximately 10 μg of purified RNA was used to prepare double-stranded cDNA (Supercript GIBCO/BRL, Rockville, MD) using a T7 (dT)24 primer containing a T7 RNA polymerase promoter binding site.
1) first led us to investigate whether DEK in nuclear extracts binds to MHC class II Y-box regulatory elements in vitro . With a double-stranded oligonucleotide probe containing the HLA-DQA1*0101 Y-box sequence, EMSAs revealed similar binding activity in nuclear extracts from B lymphoid (Namalwa), T lymphoid (Jurkat, CEM), monocytoid (U937), and HeLa cell lines (Fig.
For each brain region, 1 μg poly(A) +RNA was converted to double-stranded T7 cDNA.