Example sentences for: double-stranded

How can you use “double-stranded” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • Double-stranded cDNA product was purified via phenol-chloroform extraction, and then used as template in first round of in vitro transcription (IVT) of cRNA.

  • This result indicated that with regard to the mechanism of E. coli topoisomerase I, the ZD domain was likely to function as a single-stranded DNA binding domain instead of having double-stranded DNA binding function as previously suggested [ 21 ] . Even though Zn(II) binding transcription factors that recognise specific double-stranded DNA are well represented in eukaryotes [ 23 24 ] , there are also numerous examples of Zn(II) coordination being required for interaction with single-stranded nucleic acid or damaged DNA with single-strand characteristics [ 24 25 26 27 ] .

  • Approximately 10 μg of purified RNA was used to prepare double-stranded cDNA (Supercript GIBCO/BRL, Rockville, MD) using a T7 (dT)24 primer containing a T7 RNA polymerase promoter binding site.

  • 1) first led us to investigate whether DEK in nuclear extracts binds to MHC class II Y-box regulatory elements in vitro . With a double-stranded oligonucleotide probe containing the HLA-DQA1*0101 Y-box sequence, EMSAs revealed similar binding activity in nuclear extracts from B lymphoid (Namalwa), T lymphoid (Jurkat, CEM), monocytoid (U937), and HeLa cell lines (Fig.

  • For each brain region, 1 μg poly(A) +RNA was converted to double-stranded T7 cDNA.


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