Words similar to double-stranded
Example sentences for: double-stranded
How can you use “double-stranded” in a sentence? Here are some example sentences to help you improve your vocabulary:
On the basis of the known crystal structure of T7 RNA polymerase bound to a 17 base-pair, double-stranded promoter sequence, it appears that T7 promoter recognition occurs largely through direct or water-mediated hydrogen bonding [ 2 ] . The identities of at least some of the selected mutants can be rationalized on the basis of the known protein:DNA contacts.
Approximately 10 μg of purified RNA was used to prepare double-stranded cDNA (Supercript GIBCO/BRL, Rockville, MD) using a T7 (dT)24 primer containing a T7 RNA polymerase promoter binding site.
In positive controls, A549 cell treated with calcium mobilizers (thapsigargin or A23178) did increase eIF-2 phosphorylation, as has been shown earlier [ 30 ] . These results provide the first direct evidence that the 21-nt long double-stranded siRNAs fail to trigger interferon response in mammalian cells, and hence, can be used as specific antiviral agents.
These authors introduced the double-stranded RNA by electroporation also, using conditions very similar to ours.
RNA from the sample is converted to double-stranded cDNA and then labeled by in vitro transcription with biotinylated nucleotides.
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