Example sentences for: double-stranded

How can you use “double-stranded” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • Approximately 10 μg of purified RNA was used to prepare double-stranded cDNA (Supercript GIBCO/BRL, Rockville, MD) using a T7 (dT)24 primer containing a T7 RNA polymerase promoter binding site.

  • The ZD domain is not required for catenation of double-stranded DNA circles

  • Amplified products were diluted 10-fold in sterile water and 1 μl of diluted primary PCR products were added to 24 μl of secondary PCR master mix containing nested primers, 1 and 2R, to ensure specific amplification of double-stranded templates containing both adaptors.

  • Before moving on, a quick course in molecular biology: All living creatures, from viruses on up, pull off the feat of self-replication by encoding the necessary information in nucleic acids--in particular the double-stranded DNA discovered by Crick and Watson or, for a few renegade viruses, single-stranded RNA or even single-stranded DNA.

  • The cDNA microarray represents a popular array type in which double-stranded PCR products amplified from expressed sequence tag (EST) clones are spotted onto glass slides [ 7, 8], allowing gene-expression profiles to be determined with high reproducibility and efficiency.


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