Words similar to double-stranded
Example sentences for: double-stranded
How can you use “double-stranded” in a sentence? Here are some example sentences to help you improve your vocabulary:
For example, RP L18, which was significantly down-regulated by NGF, has been reported to negatively regulate double-stranded RNA (dsRNA)-activated protein kinase (PKR) [ 45 ] . As an additional example RP S19, which is also down-regulated by NGF, has been implicated in erythropoiesis and has also been described as a monocyte chemotactic factor [ 46 ] . As one last example, rat RP L9 which we found to be up-regulated by NGF starting within a few hours and maximally by 8 hrs, has been shown to be the ortholog of the yeast gene grc5 which appears to be involved in multiple cellular functions including growth control, cytoskeleton control and energy metabolism [ 47 ] . Identification of NGF-regulated RPs should now facilitate exploration of their potential roles in the responses of cells to neurotrophins.
Double-stranded cDNA was made and biotinylated-cRNA synthesized using T7 polymerase.
Amplified products were diluted 10-fold in sterile water and 1 μl of diluted primary PCR products were added to 24 μl of secondary PCR master mix containing nested primers, 1 and 2R, to ensure specific amplification of double-stranded templates containing both adaptors.
The cDNA microarray represents a popular array type in which double-stranded PCR products amplified from expressed sequence tag (EST) clones are spotted onto glass slides [ 7, 8], allowing gene-expression profiles to be determined with high reproducibility and efficiency.
Briefly, total cellular RNA was prepared with Qiagen RNeasy reagents, and 5 μg samples were used to prepare T7-dT-primed double-stranded cDNA (SuperScript Choice, Invitrogen).
Loading...